Depletion of CD4+CD25+CD127lo regulatory T cells does not increase allergen-driven T cell activation
Article first published online: 3 AUG 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 38, Issue 11, pages 1752–1759, November 2008
How to Cite
Skrindo, I., Farkas, L., Kvale, E. O., Johansen, F.-E. and Jahnsen, F. L. (2008), Depletion of CD4+CD25+CD127lo regulatory T cells does not increase allergen-driven T cell activation. Clinical & Experimental Allergy, 38: 1752–1759. doi: 10.1111/j.1365-2222.2008.03081.x
- Issue published online: 22 OCT 2008
- Article first published online: 3 AUG 2008
- Submitted 12 December 2007; revised 25 May 2008; accepted 19 June 2008
- allergic rhinitis;
- regulatory T cells;
- T cells;
- Th2 cytokines
Background It has been suggested that allergic diseases are caused by defective suppression of allergen-specific Th2 cells by CD4+CD25+ regulatory T cells. However, such studies have been hampered by the difficulty in distinguishing regulatory T cells from CD25-expressing activated T cells. Recently, it was shown that conventional T cells expressed high levels of CD127, whereas regulatory T cells were CD127lo, allowing discrimination between these distinct T cell subpopulations.
Objective The aim of this study was to study whether the putative regulatory subset defined as CD4+CD25+CD127lo was involved in grass pollen-reactive T cell responses.
Methods Peripheral blood mononuclear cells (PBMCs) were obtained from allergic donors and non-atopic controls out of season. Grass pollen-induced cytokine production and proliferation were compared in cultures of undepleted cells and cells depleted of CD4+CD25+, CD4+CD25+CD127hi or CD4+CD25+CD127lo T cells.
Results Undepleted cell cultures from allergic patients showed significantly increased proliferation and Th2 cytokine production compared with non-atopic controls. Depletion of all CD25+ T cells did not increase cytokine production or proliferation, and more importantly, no increase in Th2 cytokine production or proliferation was observed in cell cultures depleted of CD4+CD25+CD127lo cells (putative regulatory T cells) compared with undepleted PBMCs in both the allergic and the non-atopic group.
Conclusion Our study showed that T cells from grass pollen-allergic patients and non-atopic controls responded very differently to grass pollen extract, but this difference could not be explained by differences in regulatory T cell function. Further studies are needed to understand the importance of regulatory T cells in allergy.