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Fig. S1. Inflammatory profiles of mice following brief allergen exposure. (a) Serum IgE levels determined by ELISA (level of detection 1.6 ng/mL). (b) Bronchoalveolar lavage (BAL) supernatant IL-13 levels (level of detection 19.5 pg/mL). (c) BAL supernatant IL-4 levels (level of detection 7.8 pg/mL). (d) BAL supernatant IFN-γ levels (level of detection 3.1 pg/mL). Individual data points are presented, as a large majority of samples were below manufacturer recommended level of detection, and a bar graph could not be generated. (e) BAL eosinophil counts (× 104 cells). (f) Tissue eosinophil counts (eosinophils/μm2). BALB/c saline: open white bar; BALB/c ovalbumin (OVA): hatched white bar; C57Bl/6 saline: open white bar; C57Bl/6 OVA: solid black bar. Data are expressed as mean (SEM); 10 mice per group. Dashed lines represent the level of detection of ELISA kit. Asterisk (*) indicates significant difference from corresponding control animals (P<0.05).

Fig. S2. TGF-β1 gene expression in lungs from BALB/c and C57Bl/6 mice following brief allergen exposure. Gene expression was performed 2 weeks following a single 2-day period of allergen exposure (Day 34) in BALB/c mice and C57Bl/6 mice. BALB/c saline: open white bar; BALB/c ovalbumin (OVA): hatched white bar; C57Bl/6 saline: open white bar; C57Bl/6 OVA: solid black bar. Data are expressed as mean (SEM); eight mice per group.

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