1Contributed equally to this work.
Allergen extract-induced interleukin-10 in human memory B cells inhibits immunoglobulin E production
Article first published online: 6 APR 2009
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 39, Issue 5, pages 671–678, May 2009
How to Cite
Milovanovic, M., Heine, G., Zuberbier, T. and Worm, M. (2009), Allergen extract-induced interleukin-10 in human memory B cells inhibits immunoglobulin E production. Clinical & Experimental Allergy, 39: 671–678. doi: 10.1111/j.1365-2222.2009.03233.x
- Issue published online: 7 APR 2009
- Article first published online: 6 APR 2009
- Submitted 26 June 2008; revised 16 December 2008; accepted 23 January 2009
- allergen extracts;
- B cells;
Background Elevated specific IgE antibody levels are common in atopic individuals, caused by T-helper type 2-dominated B cell activation. The induction of antigen-specific IL-10 secreting T cells is discussed as an important mechanism during specific immunotherapy. By contrast the presence and function of B cell-derived IL-10 is not well defined yet.
Objective We investigated whether type-I allergen extracts induce IL-10 expression in human B cells and analysed its functional role on IgE production.
Methods Human peripheral B cells were stimulated with grass pollen, house dust mite (HDM) (Dermatophagoides pteronyssimus; Der p) and dog allergen extract. Expression of IL-10 by activated human B cells was determined by flow cytometric analysis and ELISA. Functional analysis considering immunoglobulin production was assayed by ELISA.
Results The allergen extracts studied induced IL-10 expression in B cells. However, the ability to induce IL-10 differed between the allergen extracts. The most potent allergen extract was dog (169±28 pg/mL), followed by grass pollen (141±10 pg/mL) and HDM allergen (125±11 pg/mL). Upon allergen extract stimulation only CD27 expressing memory B cells produced IL-10 and co-expressed the very early activation antigen CD69. The addition of allergen extracts to B cells activated by anti-CD40 and IL-4 selectively inhibited IgE which was dependent on allergen extract-induced IL-10. By contrast the other immunoglobulin subclasses like IgA, IgG or IgM were not altered upon allergen extract challenge.
Conclusion Our data indicate that allergen-activated memory B cells can modulate IgE production through secretion of IL-10.