Association of G-protein-coupled receptor 154 with asthma and total IgE in a population of the Caribbean coast of Colombia
Article first published online: 16 JUL 2009
© 2009 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 39, Issue 10, pages 1558–1568, October 2009
How to Cite
Vergara, C., Jiménez, S., Acevedo, N., Martínez, B., Mercado, D., Gusmão, L., Rafaels, N., Hand, T., Barnes, K. C. and Caraballo, L. (2009), Association of G-protein-coupled receptor 154 with asthma and total IgE in a population of the Caribbean coast of Colombia. Clinical & Experimental Allergy, 39: 1558–1568. doi: 10.1111/j.1365-2222.2009.03311.x
- Issue published online: 10 SEP 2009
- Article first published online: 16 JUL 2009
- Submitted 20 February 2009; revised 18 May 2009; accepted 21 May 2009
- G protein-coupled receptor 154;
- immunoglobulin E
Background G protein-coupled receptor 154 was described as an asthma susceptibility gene by positional cloning. It has been subsequently associated with asthma and other inflammatory diseases in several populations with different ethnic origin. Replication of associations adds reliability to these findings.
Objective To analyze the association of G protein-coupled receptor 154 with asthma and total and mite-specific IgE levels in a population of the Caribbean Coast of Colombia.
Methods We genotyped seven single nucleotide proteins (SNPs) in GPR154 in 475 asthmatics, 394 controls and 116 families from Cartagena, Colombia using either SnaPshot or TaqMan. Total and specific IgE against Blomia tropicalis and Dermatophagoides pteronyssinus were determined by ELISA. Hardy–Weinberg equilibrium was assessed and case–control and family-based analyses were performed to evaluate the association between the SNPs and their haplotypes and asthma and IgE. Association analyses in the case–control dataset were corrected by population stratification using 52 ancestry informative markers.
Results Allelic distribution was similar to that described in other populations. Two SNPs were associated with the same direction of the effect in both datasets. Allele A of Hopo546333 was protective for asthma (case–control OR: 0.42; 95% CI: 0.17–0.99, P=0.042; P=0.043; families Z score=−2,236; P=0.025). Similarly, allele C of rs740347 conferred low risk for asthma (OR: 0.44; 95% CI: 0.28–0.70, P=0.00017; Pc=0.00037) and total IgE (OR: 0.29; 95% CI: 0.09–0.88, P=0.015; Pc=0.030) in the case–control study and families (Z score=−3.207, P=0.0013; Z score=−3.182, P=0.0014, respectively). Haplotype CCAGGT was associated with total IgE (OR: 1.76; 95% CI: 1.14–2.71, P=0.006, Pc=0.007) in the case–controls group and CGCGGT with both phenotypes (P=0.044 and P=0.032, respectively) in families. Neither SNPs nor haplotypes were associated with levels of mite-specific IgE.
Conclusions Our findings in a sample of asthmatics from Colombia suggest a relevant role of G protein-coupled receptor 154 in the pathogenesis of asthma and allergy.