Allergic sensitization is enhanced in early life through toll-like receptor 7 activation
Version of Record online: 3 SEP 2009
© 2009 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 39, Issue 12, pages 1920–1928, December 2009
How to Cite
Phipps, S., Hansbro, N., Lam, C. E., Foo, S. Y., Matthaei, K. I. and Foster, P. S. (2009), Allergic sensitization is enhanced in early life through toll-like receptor 7 activation. Clinical & Experimental Allergy, 39: 1920–1928. doi: 10.1111/j.1365-2222.2009.03335.x
- Issue online: 13 NOV 2009
- Version of Record online: 3 SEP 2009
- Submitted 14 October 2008; revised 29 June 2009; accepted 3 July 2009
Figure S1. The age of sensitisation determines the magnitude of allergic airways inflammation to antigen exposure in later life. Mice were sensitised as neonates (within the first 24 h of life) or weanlings (28 days old) to endotoxin low OVA in the absence (shaded bars) or presence (closed bars) of ssRNA. Unlike the model employed in Fig. 2, neonatal mice did not receive a ‘booster’ OVA sensitisation on day 28. Allergen challenge occurred at 6 wk of age and samples collected at 7 wk of age. a and b) Mediastinal lymph node cells were seeded into 96 well plates at 106/well and stimulated with endotoxinlow OVA for 6 days. IL-13 and IFN-g production were measured by ELISA. Shown are the results of one experiment consisting of groups of five animals. Data are mean±s.e.m; *P<0.05.
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Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.