*Contributed equally to this work.
Maternal vitamin D intake during pregnancy increases gene expression of ILT3 and ILT4 in cord blood
Article first published online: 16 DEC 2009
© 2009 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 40, Issue 5, pages 786–794, May 2010
How to Cite
Rochat, M. K., Ege, M. J., Plabst, D., Steinle, J., Bitter, S., Braun-Fahrländer, C., Dalphin, J.-C., Riedler, J., Roponen, M., Hirvonen, M.-R., Büchele, G., Renz, H., Lauener, R., Krauss-Etschmann, S., Von Mutius, E. and the PASTURE Study group (2010), Maternal vitamin D intake during pregnancy increases gene expression of ILT3 and ILT4 in cord blood. Clinical & Experimental Allergy, 40: 786–794. doi: 10.1111/j.1365-2222.2009.03428.x
The PASTURE study group: Gertraud Weiß, Ellen Üblagger, Claudia Humer, Manuela Rußegger (Austria); Juha Pekkanen, Raija Juntunen, Reetta Tiihonen, Pekka Tiittanen, Kati Huttunen, Suvi Virtanen, Timo Kauppila, Aino Nevalainen, Sami Remes, Tomi-Pekka Tuomainen, Anne Karvonen, Mikko Lappalainen (Finland); Marie-Laure Dalphin, Dominique Vuitton, Renaud Piarroux, Gabriel Reboux, Sandrine Roussel, Bertrand Sudre (France); Michael Kabesch, Susanne Schmid, Sabina Illi, Nicola Korherr, Jon Genuneit, Richard Peter, Serdar Sel, Nicole Blümer, Petra Pfefferle (Germany); Bert Brunekreef, Ulrike Gehring, Gert Dockes (The Netherlands); Felix H. Sennhauser, Susanne Loeliger, Remo Frei, Caroline Roduit (Switzerland).
- Issue published online: 19 APR 2010
- Article first published online: 16 DEC 2009
- Submitted 22 July 2009; revised 29 October 2009; accepted 3 November 2009
- atopic disease;
- birth cohort;
- farming environment;
- ILT3 and ILT4;
- vitamin D
Background Recent studies indicate that prenatal vitamin D intake may protect against the development of atopic diseases in young children. Vitamin D has been shown to induce tolerogenic antigen-presenting cells such as dendritic cells. Whether the allergy-protective potential of prenatal vitamin D is mediated through such mechanisms is, however, unknown.
Objective To evaluate the association between prenatal vitamin D supplementation and tolerogenic antigen-presenting cells in cord blood (CB) as determined by mRNA measurement of immunoglobulin-like transcripts (ILT)3 and ILT4.
Methods A prospective multi-centre birth cohort was established in rural areas of five European countries. Information on maternal exposures including vitamin D intake was collected by questionnaires during pregnancy. The gene expression of ILT3 and ILT4 was analysed by real-time PCR in the CB of 927 children. Maternal vitamin D supplementation was assessed in Finland and France (n=349).
Results Maternal vitamin D supplementation during pregnancy was associated with an increase in the gene expression of ILT3 (P=0.012) and ILT4 (P<0.001). This association remained significant for ILT4 (P=0.020) and showed a positive trend for the gene expression of ILT3 (P=0.059) after multivariate analysis controlling for various confounders.
Conclusions Vitamin D supplementation during pregnancy may increase the mRNA levels of ILT3 and ILT4 in CB. This finding may point towards an early induction of tolerogenic immune responses by maternal vitamin D intake.
Cite this as: M. K. Rochat, M. J. Ege, D. Plabst, J. Steinle, S. Bitter, C. Braun-Fahrländer, J-C. Dalphin, J. Riedler, M. Roponen, M-R. Hirvonen, G. Büchele, H. Renz, R. Lauener, S. Krauss-Etschmann, E. von Mutius and the PASTURE Study group, Clinical & Experimental Allergy, 2010 (40) 786–794.