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Maternal vitamin D intake during pregnancy increases gene expression of ILT3 and ILT4 in cord blood

Authors


  • The PASTURE study group: Gertraud Weiß, Ellen Üblagger, Claudia Humer, Manuela Rußegger (Austria); Juha Pekkanen, Raija Juntunen, Reetta Tiihonen, Pekka Tiittanen, Kati Huttunen, Suvi Virtanen, Timo Kauppila, Aino Nevalainen, Sami Remes, Tomi-Pekka Tuomainen, Anne Karvonen, Mikko Lappalainen (Finland); Marie-Laure Dalphin, Dominique Vuitton, Renaud Piarroux, Gabriel Reboux, Sandrine Roussel, Bertrand Sudre (France); Michael Kabesch, Susanne Schmid, Sabina Illi, Nicola Korherr, Jon Genuneit, Richard Peter, Serdar Sel, Nicole Blümer, Petra Pfefferle (Germany); Bert Brunekreef, Ulrike Gehring, Gert Dockes (The Netherlands); Felix H. Sennhauser, Susanne Loeliger, Remo Frei, Caroline Roduit (Switzerland).

Correspondence:
M. Rochat, Dr von Haunersche Kinderklinik, Lindwurmstrasse 4, 80337 Munich, Germany.
E-mail: mascha.rochat@med.uni-muenchen.de

Summary

Background Recent studies indicate that prenatal vitamin D intake may protect against the development of atopic diseases in young children. Vitamin D has been shown to induce tolerogenic antigen-presenting cells such as dendritic cells. Whether the allergy-protective potential of prenatal vitamin D is mediated through such mechanisms is, however, unknown.

Objective To evaluate the association between prenatal vitamin D supplementation and tolerogenic antigen-presenting cells in cord blood (CB) as determined by mRNA measurement of immunoglobulin-like transcripts (ILT)3 and ILT4.

Methods A prospective multi-centre birth cohort was established in rural areas of five European countries. Information on maternal exposures including vitamin D intake was collected by questionnaires during pregnancy. The gene expression of ILT3 and ILT4 was analysed by real-time PCR in the CB of 927 children. Maternal vitamin D supplementation was assessed in Finland and France (n=349).

Results Maternal vitamin D supplementation during pregnancy was associated with an increase in the gene expression of ILT3 (P=0.012) and ILT4 (P<0.001). This association remained significant for ILT4 (P=0.020) and showed a positive trend for the gene expression of ILT3 (P=0.059) after multivariate analysis controlling for various confounders.

Conclusions Vitamin D supplementation during pregnancy may increase the mRNA levels of ILT3 and ILT4 in CB. This finding may point towards an early induction of tolerogenic immune responses by maternal vitamin D intake.

Cite this as: M. K. Rochat, M. J. Ege, D. Plabst, J. Steinle, S. Bitter, C. Braun-Fahrländer, J-C. Dalphin, J. Riedler, M. Roponen, M-R. Hirvonen, G. Büchele, H. Renz, R. Lauener, S. Krauss-Etschmann, E. von Mutius and the PASTURE Study group, Clinical & Experimental Allergy, 2010 (40) 786–794.

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