Attenuated Bordetella pertussis vaccine strain BPZE1 modulates allergen-induced immunity and prevents allergic pulmonary pathology in a murine model
Article first published online: 22 FEB 2010
© 2010 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 40, Issue 6, pages 933–941, June 2010
How to Cite
Kavanagh, H., Noone, C., Cahill, E., English, K., Locht, C. and Mahon, B. P. (2010), Attenuated Bordetella pertussis vaccine strain BPZE1 modulates allergen-induced immunity and prevents allergic pulmonary pathology in a murine model. Clinical & Experimental Allergy, 40: 933–941. doi: 10.1111/j.1365-2222.2010.03459.x
- Issue published online: 6 MAY 2010
- Article first published online: 22 FEB 2010
- Submitted 15 June 2009 and accepted 7 December 2009
Figure S1. H&E-stained lung sections. Original magnification ×100. Perivascular and peribronchial inflammation was evaluated as (a) mild, (b) moderate, and (c) severe. (a) Mild peribronchial inflammation (centre) surrounded by clear alveoli and cross-sectioned bronchioles (bottom left, right). (b) Moderate peribronchial inflammation surrounding bronchiole (top right, bottom right) and moderate alveolar inflammation. (c) Severe perivascular and peribronchial inflammation with bronchial epithelial hypertrophy (centre, centre right). Images represent typical pathology at each grade.
Figure S2. BPZE1 challenge prior to OVA sensitisation decreases airway hyperresponsiveness to sensitising allergen. Airway responsiveness was assessed on day 37 by methacholine induced airflow obstruction from conscious mice using whole-body plethysmography in conjunction with the BioSystem XA software (Buxco Electronics, USA) as previously described . (a) Non-sensitised (), OVA-sensitised (), BPSM infected+OVA sensitisation () and BPZE1 challenge+OVA sensitisation (). (b) Nonsensitised (Control ), OVA-sensitised (), non-sensitised BPSM infected () and nonsensitised BPZE1 challenge (). Results are expressed as mean enhanced pause (PenH)±S.E.M. Where no error bars are visible, error bars are shorter than the size of the data point symbol. Data points represent the mean PenH from eight mice in this case.
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