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Figure S1. Dose-dependence of allergen-specific Th2 cytokine expression. Ragweed- specific cytokine+ cells are expressed as a percentage of allergen-specific CD154+CD3+CD4+T cells after stimulation with different concentrations of ragweed. Representative data are shown for 3 PBMC donors.

Figure S2. Dose-dependence of allergen-specific Th2 cytokine expression. Ragweed- specific cytokine+ cells are expressed as a percentage of allergen-specific CD154+CD3+CD4+T cells after stimulation with different concentrations of ragweed. Representative data are shown for 3 PBMC donors.

Figure S3. Allergen-specific IL-4, IL-5, and IL-13 expression all strongly correlate. Cytokine+ cells are expressed as a percentage of Amb a 1- or ragweed-specific CD154+ CD3+ CD4+ T cells. Spearman rank order correlation coefficient was used to evaluate relationships between expression of IL-4, IL-5, and IL-13. Data are for all subjects pre-treatment (n=32).

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