ORIGINAL ARTICLE/Basic Mechanisms in Allergic Disease
Expression of CD203c and CD63 in human basophils: relationship to differential regulation of piecemeal and anaphylactic degranulation processes
Article first published online: 13 JUL 2010
© 2010 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 40, Issue 9, pages 1365–1377, September 2010
How to Cite
MacGlashan, D. (2010), Expression of CD203c and CD63 in human basophils: relationship to differential regulation of piecemeal and anaphylactic degranulation processes. Clinical & Experimental Allergy, 40: 1365–1377. doi: 10.1111/j.1365-2222.2010.03572.x
- Issue published online: 4 AUG 2010
- Article first published online: 13 JUL 2010
- Submitted 12 February 2010; revised 12 May 2010; accepted 14 May 2010
- activation marker;
- histamine release;
- protein kinase C
Background Activation of human basophils results in the release of many different mediators and the expression of new cell surface proteins. The markers CD63 and CD203c have been used in recent years to assess basophil activation but there have been many studies that demonstrate that expression of these markers can be dissociated from histamine release.
Objective To determine the signal transduction requirements for CD203c and CD63 expression.
Methods The current study began by exploring the dependency of CD203c and CD63 expression on protein kinase C (PKC) using known selective inhibitors of PKC.
Results Between 30 and 300 nm, Ro-31-8220 and bisindoylmaleimide II (Bis II) had no effect on formyl–met–leu–phe- or anti-IgE-induced CD63 or CD203c but enhanced IgE-mediated expression of CD63 by an average of 15-fold at concentrations >1 μm. These results led to the suggestion that these inhibitors altered the normal pathways of degranulation (by a non-PKC dependent mechanism), shifting the normal presence of piecemeal degranulation to the process termed anaphylactic degranulation (AND). Morphological studies demonstrated that concentrations of Ro-31-8220 and Bis II>1 μm dramatically increased the presence of degranulation sacs, a morphological feature of AND.
Conclusion It is proposed that CD63 expression results from only the AND form of histamine release.
Cite this as: D. MacGlashan Jr, Clinical & Experimental Allergy, 2010 (40) 1365–1377.