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Clinical & Experimental Allergy

A new approach to study exhaled proteins as potential biomarkers for asthma

Authors

  • K. Bloemen,

    1. Flemish Institute for Technological Research (VITO), Environmental Risk and Health, Toxicology, Mol, Belgium
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  • R. Van Den Heuvel,

    1. Flemish Institute for Technological Research (VITO), Environmental Risk and Health, Toxicology, Mol, Belgium
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  • E. Govarts,

    1. Flemish Institute for Technological Research (VITO), Environmental Risk and Health, Toxicology, Mol, Belgium
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  • J. Hooyberghs,

    1. Flemish Institute for Technological Research (VITO), Environmental Risk and Health, Toxicology, Mol, Belgium
    2. Department WNI, Hasselt University, Diepenbeek, Belgium
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  • V. Nelen,

    1. Provincial Institute of Hygiene (PIH), Antwerp, Belgium
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  • E. Witters,

    1. Flemish Institute for Technological Research (VITO), Environmental Analysis and Technology, Mol, Belgium
    2. Department of Biology, Center for Proteome Analysis and Mass Spectrometry (CEPROMA), University of Antwerp (UA), Antwerp, Belgium
    3. Department of Ecophysiology, Biochemistry and Toxicology (EBT), University of Antwerp (UA), Antwerp, Belgium
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  • K. Desager,

    1. Department of Pediatrics, University Hospital of Antwerp (UZA), Edegem, Belgium
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  • G. Schoeters

    1. Flemish Institute for Technological Research (VITO), Environmental Risk and Health, Toxicology, Mol, Belgium
    2. Department of Biomedical Sciences, University of Antwerp (UA), Antwerp, Belgium
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Correspondence:
Greet Schoeters, Flemish Institute for Technological Research (VITO), Environmental Risk and Health, Toxicology, Boeretang 200, 2400 Mol, Belgium.
E-mail: greet.schoeters@vito.be

Abstract

Cite this as: K. Bloemen, R. Van Den Heuvel, E. Govarts, J. Hooyberghs, V. Nelen, E. Witters, K. Desager and G. Schoeters, Clinical & Experimental Allergy, 2011 (41) 346–356.

Summary

Background Asthma is a complex clinical disease characterized by airway inflammation. Recently, various studies reported on the analysis of exhaled breath condensate (EBC) in the search for potential biomarkers for asthma. However, in a complex disease such as asthma, one biomarker might not be enough for early diagnosis or follow-up.

Objective The use of proteome analysis may reveal disease-specific proteolytic peptide or protein patterns, and may lead to the identification of novel proteins for the detection of asthma.

Methods Liquid chromatography and mass spectrometry were used to separate and detect proteins (proteolytic peptides) present in EBC samples from 30 healthy children and 40 children with asthma in the age group of 6–12 years.

Results Support vector machine analysis resulted in differentiating profiles based on asthma status. These proteolytic peptide patterns were not correlated to some well known (spirometry, exhaled nitric oxide) and more recently described exhaled markers (EBC pH, LTB4). The more abundant proteins in EBC were identified as cytokeratins, albumin, actin, haemoglobin, lysozyme, dermcidin, and calgranulin B.

Conclusion Although the exact role in the disease development or physiological state of the airways of the proteins described in the presented pattern is not clear at this moment, this is an important step in the search for exhaled biomarkers for asthma. This study shows that EBC contains proteins that are of interest for future non-invasive asthma diagnosis or follow-up.

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