In vitro diagnosis of T cell-mediated drug allergy
Article first published online: 22 FEB 2011
© 2011 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 41, Issue 4, pages 461–470, April 2011
How to Cite
Porebski, G., Gschwend-Zawodniak, A. and Pichler, W. J. (2011), In vitro diagnosis of T cell-mediated drug allergy. Clinical & Experimental Allergy, 41: 461–470. doi: 10.1111/j.1365-2222.2011.03701.x
- Issue published online: 13 MAR 2011
- Article first published online: 22 FEB 2011
Cite this as: G. Porebski, A. Gschwend-Zawodniak and W. J. Pichler, Clinical & Experimental Allergy, 2011 (41) 461–470.
Diagnosis of drug hypersensitivity relies on history, skin tests, in vitro tests and provocation tests. In vitro tests are of great interest, due to possible reduction of drug provocation tests. In this review we focus on best investigated in vitro techniques for the diagnosis of T cell-mediated drug hypersensitivity reactions. As drug hypersensitivity relies on different pathomechanisms and as a single diagnostic test usually does not cover all possible reactions, it is advisable to combine different tests to increase the overall sensitivity. Recently, proliferation-based assays have been supplemented by a panel of novel in vitro tests including analysis of cytotoxic potential of effector cells (granzyme B, granulysin, CD107a), evaluation of cytokine secretion (IL-2, IL-5, IL-13, and IFN-γ) and up-regulation of cell surface activation markers (CD69). We discuss the latest findings and readout systems to identify causative drugs by detecting functional and phenotypic markers of drug-reacting cells, and their ability to enable a more conclusive diagnosis of drug allergy.