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Figure S1. Amino acid composition of CDRH3 of IgE as assessed by analysis of transcripts in mucosal tissue of subjects diagnosed with NAFES (green bars) and (blue bars) in comparison with the amino acid composition of CDRH3 in transcripts found in peripheral blood mononuclear that encode IgE (yellow bars (PBMC-IgE-W[1]) and red bars (PBMC IgE-K [2])) or IgM (white bars (PBMC IgM-K [2])). Amino acids are defined by the standard one-letter code. No extensive misrepresentation of residues is seen in IgE-encoding transcriptomes in NAFES and CRS.

Figure S2. Different levels of mutations in different PCR-products with an origin in three different immunoglobulin gene rearrangements (clonotypes) identified from patients 7 (A) and 11 (B), both diagnosed with NAFES, and patient 5, diagnosed with CRS (C). CDR, as defined by the IMGT nomenclature [3], are highlighted with black lines. Note the presence of substitutions shared by all members of the clonetypes derived from subjects 7 (A) and 11 (B) in comparison to their germline gene origin (highlighted by stars), as well as substitutions differentiating the different sequences in all three clonotypes, each with a likely origin in a common B lymphocyte rearrangement. The grey line indicates the part of the amplified sequences that encodes the constant domain and thus defines that these clonotypes have been amplified from the IgE-encoding transcriptome.

Table S1. Compilation of allergy diagnostic tests performed on subjects included in this study.

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