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Fig. S1. Inactivation of Havcr2 (Tim3) by homologous recombination (A) The structure of the Tim3 locus, the targeting vector, and predicted homologous recombination event. (B) Genotyping of genomic DNA from Tim3 gene-targeted mice by PCR analysis. (C) RT-PCR analysis of Tim3 expression, assessed using exon-specific primer sets in wild type and Tim3−/− Th1 and Th2 cells. Con, vector containing full length Tim3 transcript; Neg, negative control. (D) Flow cytometric analysis of Tim3 expression on lung cell suspensions from OVA sensitised wild type and Tim3−/− mice.

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