*Current Address: Era's Lucknow Medical College and Hospital, Sarfarazganj, Hardoi Road, Lucknow 226003, UP, India.
Potential allergens of green gram (Vigna radiata L. Millsp) identified as members of cupin superfamily and seed albumin
Version of Record online: 1 JUN 2011
© 2011 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 41, Issue 8, pages 1157–1168, August 2011
How to Cite
Misra, A., Kumar, R., Mishra, V., Chaudhari, B. P., Raisuddin, S., Das, M. and Dwivedi, P. D. (2011), Potential allergens of green gram (Vigna radiata L. Millsp) identified as members of cupin superfamily and seed albumin. Clinical & Experimental Allergy, 41: 1157–1168. doi: 10.1111/j.1365-2222.2011.03780.x
- Issue online: 13 JUL 2011
- Version of Record online: 1 JUN 2011
- Submitted 11 August 2010; revised 5 April 2011; accepted 8 April 2011
Figure S1. Analysis of conserved domain of Vig r3 using Blast scarch. Vig r3 shares conserved domain with Cupin superfamily.
Figure S2. ELISA inhibition of green gram with extract of green gram soyabean red gram and chickpea using green gram CPE on solid phase. Pooled sera of Green gram allergic patient were used. Mean (n±3) and SEs (bars) are represented.
Figure S3. Experimental design. Three groups of BALB/c mice (n=15/group) were sensitized once a week either with 100 μL (1 mg/mL) of peanut or green gram CPE or PBS intraperitoneally. Blood samples were collected on days 15, 43 and 59. A subgroup of these mice (n=5/group) and their blood/spleen collected for RT-PCR analysis after challenge with 10 mg of CPE for cytokines mRNA. On day 60, remaining (n 5/group) mice were challenged and symptoms were scored. Lung and spleen tissues were collected for histopathology. *Blood sampling for specific IgE, IgG1 and sera for 2DE immunoblot (day 43).
Table S1. Sequence homology analysis of tryptic fragments of IgE-binding green gram proteins.
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Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.