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Staphylococcal enterotoxin B compromises the immune tolerant status in the airway mucosa

Authors

  • T. Liu,

    Corresponding author
    • Department of Otolaryngology, Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China
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    • These authors equally contributed to this work and are joint-first authors.
  • X. Liang,

    1. Department of Otolaryngology, Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China
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    • These authors equally contributed to this work and are joint-first authors.
  • T.-L. Li,

    1. Department of Otolaryngology, Shanxi Provincial People's Hospital, Taiyuan, China
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    • These authors equally contributed to this work and are joint-first authors.
  • J. Ma,

    1. Department of Otolaryngology, Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China
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  • J.-F. Yang,

    1. Department of Otolaryngology, Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China
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  • P.-C. Yang

    Corresponding author
    • Department of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, Canada
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Correspondence: Dr Ping-Chang Yang, Room 3303, 50 Charlton Ave East, Hamilton, ON, Canada L8N 4A6. E-mail: yangp@mcmaster.ca and Dr Tao Liu, Department of Otolaryngology, Head & Neck Surgery, The First Hospital, Shanxi Medical University, Taiyuan, China. E-mail: sylt2009@126.com

Summary

Background

The breakdown of immune tolerance plays a critical role in allergic disorders; the mechanism of breaching immune tolerance remains largely unknown.

Objective

The present study aimed to investigate the role of Staphylococcal enterotoxin B (SEB) in the interference of the immune tolerance in the nasal mucosa.

Methods

The immune tolerant components, tolerogenic dendritic cells (TolDC) and regulatory T cells (Treg), were assessed in the surgically removed nasal mucosa from patients with allergic rhinitis (AR) or non-AR chronic rhinitis. The contents of SEB and integrin alphavbeta6 (avb6) in the nasal epithelium were assessed using enzyme-linked immunoassay. The ability of avb6 on TolDC induction and the effect of SEB on suppression of avb6 in nasal epithelial cells were observed in cell culture.

Results

Compared with that in the non-AR nasal mucosa, the frequencies of TolDCs/Tregs were lower, the contents of SEB were higher and the contents of avb6 were lower in the AR nasal mucosa. Avb6 had the ability to induce the development of TolDCs in vitro; the latter had the ability to induce Treg development. The expression of avb6 was detected in nasal epithelial cells in culture that could be suppressed by SEB.

Conclusions and clinical relevance

The components of immune tolerance machinery, TolDCs and Tregs were suppressed in the AR nasal mucosa. The increases in SEB and decreases in avb6 in nasal epithelium are associated with the compromises of immune tolerance in the nasal mucosa. SEB has the ability to suppress the expression of avb6 in nasal epithelial cells.

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