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cea3889-sup-0001-FigureS1.docWord document1986KFigure S1. Detection of CXCR4 expression on cord blood-derived HPC and eosinophil-lineage committed progenitor cells by sequential multi-gating flow cytometry. Plots 1–4 represent staining of CD34-enriched population of cord blood-derived progenitor cells stained with CD45 FITC/CD34APC. True CD34+ blast cells were identified as cells with CD45low/CD34high staining and low forward (FSC) and side scatter (SSC) (Plots 1–4), as previously described [1]. Events in region R4 (true progenitor cells) were further analysed for staining with PerCp-linked isotype control or CXCR4 mAb (Plots 5 and 6). Data were collected as the number of positive cells at the 99% confidence limit (i.e. relative to a marker set to include only 1% of cells stained with control antibody). Data show 65.35% of cord blood-derived CD34+45+ cells express CXCR4. Events in region R4 (true progenitor cells) were also analysed for staining with PE-linked isotype control or IL-5Rα mAb (Plots 7 and 8). Region 5 identified CD34+45+IL-5Rα+ (Plot 8: eosinophil-lineage committed progenitors) which were then further analysed for expression of PerCp-linked isotype control or CXCR4 mAb (Plots 9 and 10). Data were collected as the number of positive cells at the 99% confidence limit (i.e. relative to a marker set to include only 1% of cells stained with control antibody). Data show 66.97% of cord blood-derived CD34+45+ cells express CXCR4.
cea3889-sup-0002-FigureS2.docWord document986KFigure S2. Expression of CCR3 on HPC. Events in region R4 (true progenitor cells) were further analysed for staining with APC-linked isotype control or CCR3 mAb (Plots 1 and 2). Data were collected as the number of positive cells at the 99% confidence limit (i.e. relative to a marker set to include only 1% of cells stained with control antibody). Data show 88.24% of cord blood-derived CD34+45+ cells express CCR3.
cea3889-sup-0003-FigureS3.docWord document1023KFigure S3. Expression of IL-4Rα on HPC and eosinophil-lineage committed progenitors. From the previous figure, events in region R4 (HPC cells; Plots 1 and 2) and R5 (eosinophil-lineage committed progenitors; Plots 3 and 4) were analysed for staining with PerCp-linked isotype control or IL-4Rα mAb. Data were collected as the number of positive cells at the 99% confidence limit (i.e. relative to a marker set to include only 1% of cells stained with control antibody). Data show 1.897% of cord blood-derived HPC (CD34+45+) and 1.43% of eosinophil-lineage committed progenitors express IL-4Rα.
cea3889-sup-0004-FigureS4.docWord document1062KFigure S4. Expression of IL-13Rα1 on HPC and eosinophil-lineage committed progenitors. From the previous figure, events in region R4 (HPC cells; Plots 1 and 2) and R5 (eosinophil-lineage committed progenitors; Plots 3 and 4) were analysed for staining with PerCp-linked isotype control or IL-13Rα1 mAb. Data were collected as the number of positive cells at the 99% confidence limit (i.e. relative to a marker set to include only 1% of cells stained with control antibody). Data show 2.33% of cord blood-derived HPC (CD34+45+) and 0.91% of eosinophil-lineage committed progenitors express IL-13Rα1.
cea3889-sup-0005-Supplementaldata.docWord document36K 

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