12-hydroxy-eicosatetraenoic acid (12-HETE): a biomarker of Churg-Strauss syndrome
Article first published online: 15 MAR 2012
© 2011 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 42, Issue 4, pages 513–522, April 2012
How to Cite
Cite this as: W. Szczeklik, M. Sanak, L. Mastalerz, B. M. Sokołowska, A. Gielicz, J. Soja, J. Kumik, J. Musiał and A. Szczeklik, Clinical & Experimental Allergy, 2012 (42) 513–522.
- Issue published online: 15 MAR 2012
- Article first published online: 15 MAR 2012
- Accepted manuscript online: 13 DEC 2011 10:55AM EST
- Manuscript Accepted: 29 NOV 2011
- Manuscript Revised: 28 OCT 2011
- Manuscript Received: 28 JUL 2011
- Iuventus Plus 2012
- Swiss Contribution. Grant Number: PSPB – 072/2010
- Churg-Strauss syndrome;
- exhaled breath condensate;
Churg-Strauss syndrome (CSS) shares similarities with asthma and hypereosinophilic syndrome (HES). Eicosanoids – important inflammatory and signaling molecules – are present in exhaled breath condensate (EBC) and broncho-alveolar lavage fluid (BALF).
To assess eicosanoid profile both in EBC and BALF of CSS subjects searching for a pattern characteristic of this syndrome.
EBCs from 23 CSS patients, 30 asthmatics, 12 HES patients and 54 healthy controls (HC) were assessed quantitatively for 19 eicosanoids by a high-performance liquid chromatography - tandem mass spectrometry (HPLC-MS/MS). In addition, in 21 of 23 CSS subjects and in nine asthmatics, eicosanoids were determined in BALF.
EBC from CSS patients showed markedly elevated levels of 12-HETE as compared with other studied groups. BALF was characterized by a significant elevation of 12-HETE and its metabolite 12-tetranor HETE in CSS as compared with asthma. Clinical activity of CSS correlated with 12-HETE and its metabolites levels in BALF, but not in EBC.
Conclusion and Clinical Relevance
CSS is clearly distinguished from bronchial asthma, and HES by a marked increase in 12-HETE concentration in both EBC and BALF. This points to a possible new pathogenic mechanism in CSS and may help in future in establishing the diagnosis of CSS.