The PASTURE Study Group: Maija-Riitta Hirvonen, Sami Remes and Pekka Tiittanen (Finland); Marie-Laure Dalphin (France); Martin Depner, Markus Ege, Jon Genuneit, Sabina Illi, Michael Kabesch, Bianca Schaub and Juliane Weber (Germany); Remo Frei, Roger Lauener, George Loss and Caroline Roduit (Switzerland)
Exposure to microbial agents in house dust and wheezing, atopic dermatitis and atopic sensitization in early childhood: a birth cohort study in rural areas
Article first published online: 16 JUL 2012
© 2012 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 42, Issue 8, pages 1246–1256, August 2012
How to Cite
Karvonen, A. M., Hyvärinen, A., Gehring, U., Korppi, M., Doekes, G., Riedler, J., Braun-Fahrländer, C., Bitter, S., Schmid, S., Keski-Nisula, L., Roponen, M., Kaulek, V., Dalphin, J.-C., Pfefferle, P. I., Renz, H., Büchele, G., von Mutius, E., Pekkanen, J. (2012), Exposure to microbial agents in house dust and wheezing, atopic dermatitis and atopic sensitization in early childhood: a birth cohort study in rural areas. Clinical & Experimental Allergy, 42: 1246–1256. doi: 10.1111/j.1365-2222.2012.04002.x
- Issue published online: 16 JUL 2012
- Article first published online: 16 JUL 2012
- Accepted manuscript online: 21 MAR 2012 10:09AM EST
- Manuscript Accepted: 14 MAR 2012
- Manuscript Revised: 13 MAR 2012
- Manuscript Received: 1 JUL 2011
- European Union. Grant Number: QLK4-CT-2001-00250
- Graduate School in Environmental Health
- Farmers’ Social Insurance Institution
- Academy of Finland. Grant Number: 139021
- National Institute for Health and Welfare
- atopic dermatitis;
- respiratory disease;
- specific IgE
Early-life exposure to environmental microbial agents may be associated with development of wheezing and allergic diseases.
To assess the association of microbial exposure in rural homes with the risk of asthma, wheezing, atopic dermatitis and sensitization.
Birth cohorts of rural children (n = 1133), half from farmer families, were followed up from birth to 2 years of age by questionnaires in five European centres. Endotoxin and extracellular polysaccharides (EPS) of Penicillium and Aspergillus spp. were determined from living room floor and mother's mattress dust samples collected at 2 months of age. Specific IgE against 19 allergens was measured at 1 year of age. Discrete-time hazard models, generalized estimations equations (GEE) and logistic regression were used for statistical analyses.
The incidence of asthma was inversely associated with the amount of dust (adjusted odds ratio (aOR) 0.73, 95% CI 0.58–0.93) and the loads (units/m2) of EPS (aOR 0.75, 95% CI 0.55–1.04) and endotoxin (aOR 0.79, 95% CI 0.60–1.05) in the mother's mattress. Similar associations were seen with wheezing and with living room floor dust. The microbial markers were highly correlated and their effects could not be clearly separated. The inverse associations were seen especially among non-farmers. The risk of sensitization to inhalant allergens increased with increasing endotoxin exposure from mattress dust. No associations were observed with concentrations (units/g) or with atopic dermatitis.
Conclusion and Clinical Relevance
The amount and microbial content of house dust were inversely associated with asthma and wheezing, but due to high correlations between microbial agents and amount of dust, it was not possible to disentangle their individual effects. New ways to better measure and represent exposure to environmental microbes, including indexes of biodiversity, are needed especially among farmers.