Monitoring neutrophils and platelets during casein-induced anaphylaxis in an experimental BALB/c mouse model
Article first published online: 15 JUN 2012
© 2012 Blackwell Publishing Ltd
Clinical & Experimental Allergy
Volume 42, Issue 7, pages 1119–1128, July 2012
How to Cite
Cite this as: D. Krishnamurthy, P. Starkl, K. Szalai, F. Roth-Walter, S. C. Diesner, M. Mittlboeck, C. Mannhalter, E. Untersmayr and E. Jensen-Jarolim, Clinical & Experimental Allergy, 2012 (42) 1119–1128.
- Issue published online: 15 JUN 2012
- Article first published online: 15 JUN 2012
- Accepted manuscript online: 9 APR 2012 08:48AM EST
- Manuscript Accepted: 6 FEB 2012
- Manuscript Revised: 3 FEB 2012
- Manuscript Received: 10 JUN 2011
- Austrian Science Fund. Grant Number: #APW01205FW
- SFB. Grant Number: F1808-B013
- Biomedical International R+D
- food allergy;
- IgE-independent mechanism;
- neutrophils platelets
With respect to the cellular players, mast cells and basophils have been well studied in experimental murine systemic anaphylaxis models, but the role of neutrophils and platelets is not fully understood today.
We tested the hypothesis that neutrophils and platelets might participate in an antigen-induced anaphylaxis model.
BALB/c mice were sensitized intraperitoneally with alum-adsorbed casein. A period of 2 weeks later, mice were challenged with 100 μg casein intravenously and immediate hypersensitivity reactions were assessed by rectal temperature measurements and monitoring the physical activity. Subsequently, leucocytes were counted in the peripheral blood as well as quantified in situ in typical shock organs like lung, liver and spleen, heart and kidney.
Mice sensitized with casein showed casein-specific IgG1, IgE, and IgG2a. When sensitized mice were specifically challenged with casein they developed immediate hypersensitivity reactions including drop of temperature and reduced activity. Furthermore, pronounced peripheral neutropenia and reduced platelet counts correlated with the severity of the hypersensitivity reactions. In the histological analyses of collected tissues we observed lung interstitial neutrophilia using Gr-1 staining. These events occurred specifically in mice sensitized and challenged with casein, in contrast to control groups.
On the basis of our data we suggest that in addition to mast cells and basophils, neutrophils and platelets participate in the anaphylactic response in this BALB/c mouse model. Platelet and neutrophils expressing relevant immunoglobulin receptors may therefore have a synergistic effect with allergen specific IgE as well as IgG antibodies in food-induced anaphylaxis. We suggest that management of these cells could be of clinical importance to handle anaphylaxis.