Conflict of interest: none declared.
Experimental dermatology •Original article
Automicroneedle therapy system combined with topical tretinoin shows better regenerative effects compared with each individual treatment
Article first published online: 11 JUN 2012
© The Author(s). CED © 2012 British Association of Dermatologists
Clinical and Experimental Dermatology
Volume 38, Issue 1, pages 57–65, January 2013
How to Cite
Kim, J. H., Park, H. Y., Jung, M. and Choi, E. H. (2013), Automicroneedle therapy system combined with topical tretinoin shows better regenerative effects compared with each individual treatment. Clinical and Experimental Dermatology, 38: 57–65. doi: 10.1111/j.1365-2230.2012.04405.x
- Issue published online: 18 DEC 2012
- Article first published online: 11 JUN 2012
- Accepted for publication 6 January 2012
Background. Regenerative therapy is a relatively new dermatological field. However, the currently available topical agents are unsuitable for transdermal drug delivery because of their high molecular weight and low liposolubility. Therefore, a more effective transdermal drug delivery system is needed in order to achieve better therapeutic effects with these agents. A recently introduced microneedle therapy system (MTS), which is a mechanical method for making minute holes in the skin, improves transdermal delivery. A recently developed refinement of this technique, the automicroneedle therapy system (AMTS), has several advantages over the traditional MTS, as it can achieve consistent results because of its automatic punching method.
Aim. To evaluate the cutaneous effects of an AMTS in combination with topical tretinoin.
Methods. Twelve hairless mice were divided into two groups, and the dorsal skin of each mouse was marked down the centre. The first group was treated with the AMTS plus 0.025% tretinoin on one side of the back, and with 0.025% tretinoin only on the other side. The other group was treated with the AMTS and vehicle on one side, while the other side was left untreated. The effects on cutaneous regeneration and the treatment side-effects were evaluated by functional assessment including transepidermal water loss and skin hydration, and by histopathology including epidermal and dermal thickness, and density of collagen fibres. Western blotting and real-time reverse transcriptase PCR were also performed to determine protein and mRNA expression of procollagen type 1 and matrix metalloproteinase-13.
Results. Compared with the individual treatments (the AMTS alone or tretinoin alone) the combination of tretinoin plus the AMTS produced greater dermal regeneration as a result of increased proliferation of collagen fibres. This combination therapy did not result in treatment-related adverse effects.
Conclusions. An AMTS combined with topical tretinoin is a safe and effective method for skin regeneration, which works by increasing collagen production, and might be a new therapeutic option for regenerative therapy.