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Keywords:

  • cationic;
  • high affinity;
  • anti-DNA;
  • lupus nephritis

SUMMARY

To investigate differences between cationic anti-dsDNA antibodies during active and inactive nephritis, low- and high-affinity IgG anti-dsDNA antibodies were prepared from sera of a lupus patient and compared for their binding affinity, spectrotype, and idiotype expression. The ratio of high-affinity to low-affinity anti-DNA antibodies and the relative avidity of the high-affinity anti-DNA antibodies decreased when active nephritis became inactive. Isoelectric focusing showed that cationic anti-dsDNA populations were present predominantly in the high-affinity fraction during active nephritis and in the low-affinity fraction during inactive nephritis. Idiolypic analysis by ELISA and Western blotting showed that the high-affinity cationic anti-DNA antibodies during active nephritis were idiotypically different from their low-affinity counterparts during inactive nephritis. The differences in binding affinity and idiotypy of the cationic anti-dsDNA antibodies suggest that certain serum IgG anti-dsDNA antibodies with both cationic charge and high affinity may be associated with active nephritis.