1365-2249/asset/cei_left.gif?v=1&s=ffee144c532fe5e73bbb89290ba14e154b0d400b)
1365-2249/asset/cei_right.gif?v=1&s=c14d545a5215173707c8202758d5b25fd0bede44)
Effects of alveolar macrophage depletion on liposomal vaccine protection against respiratory syncytial virus (RSV)
Article first published online: 1 JUN 2006
DOI: 10.1111/j.1365-2249.2006.03114.x
Additional Information
How to Cite
Benoit, A., Huang, Y., Proctor, J., Rowden, G. and Anderson, R. (2006), Effects of alveolar macrophage depletion on liposomal vaccine protection against respiratory syncytial virus (RSV). Clinical & Experimental Immunology, 145: 147–154. doi: 10.1111/j.1365-2249.2006.03114.x
Publication History
- Issue published online: 1 JUN 2006
- Article first published online: 1 JUN 2006
- Accepted for publication 21 April 2006
- Abstract
- Article
- References
- Cited By
Keywords:
- inflammation in RSV disease;
- respiratory syncytial viral G protein vaccine;
- vaccine-enhanced RSV disease
Summary
Little is known about the identities and roles of antigen-presenting cells upon exposure to antigens of respiratory syncytial virus (RSV). Here, we focused on elucidating the importance of alveolar macrophages in conferring protective immunity in mice administered a liposome-encapsulated recombinant fragment of the RSV G protein. Mice were depleted of alveolar macrophages by intranasal inoculation of liposome-encapsulated dichloromethylenediphosphonic acid (DMDP). Mice depleted of alveolar macrophages prior to immunization developed reduced levels of serum RSV-neutralizing antibody and showed dramatically impaired protection against RSV challenge. The severity of interstitial inflammation was also markedly reduced in macrophage-depleted mice. In conclusion, this study demonstrates a pivotal role for alveolar macrophages during exposure to liposome-encapsulated RSV antigen in initiating both protective and histopathological responses against RSV.