These authors contributed equally to this study.
The interleukin-10 inducing effect of transforming growth factor-β on human naive CD4+ T cells from cord blood is restricted to the TH1 subset
Article first published online: 20 DEC 2006
Clinical & Experimental Immunology
Volume 147, Issue 2, pages 352–358, February 2007
How to Cite
Kapitein, B., Tiemessen, M. M., Liu, W. M., Van Ieperen-van Dijk, A. G., Hoekstra, M. O., Van Hoffen, E. and Knol, E. F. (2007), The interleukin-10 inducing effect of transforming growth factor-β on human naive CD4+ T cells from cord blood is restricted to the TH1 subset. Clinical & Experimental Immunology, 147: 352–358. doi: 10.1111/j.1365-2249.2006.03282.x
- Issue published online: 5 JAN 2007
- Article first published online: 20 DEC 2006
- Accepted for publication 13 November 2006
- CD4+ T cells;
- immune regulation;
- naive cells;
- regulatory T cells
Transforming growth factor (TGF-β) seems to play a role in the regulation of immune responses, mainly by its suppressive function towards cells of the immune system. However, both in mice and human, conflicting data are published on the capacity of TGF-β to induce interleukin (IL)-10 secretion in both naive and skewed T cell populations. Our aim was to test the IL-10-inducing capacity of TGF-β in both naive and skewed cord blood mononuclear cells (CBMCs) and elucidate the mechanism by which TGF-β exerts its effect. Therefore, naive CBMCs and CBMCs during skewing under T helper 1 (Th1) and Th2 polarizing conditions were stimulated with CD3 and/or CD28 in the presence or absence of TGF-β. Proliferation, cytokine production and mRNA expression of transcription factors was measured. TGF-β enhanced the IL-10 production in Th1 and naive cells only, and suppressed the TH1 phenotype as demonstrated in cytokine levels and T-box expression in T cells (T-bet) expression. Interestingly, forkhead box p3 (Foxp3) expression tended to increase in both Th1 and Th2 cells. These data indicate that TGF-β can induce a regulatory phenotype in both naive and Th1-polarized cells derived from cord blood. The induction of IL-10 was not observed in Th2-polarized phenotype, indicating that TGF-β might be especially of interest for immunomodulation in Th1 cells.