These authors contributed equally to this work.
Proportion of peripheral blood and decidual CD4+ CD25bright regulatory T cells in pre-eclampsia
Article first published online: 25 APR 2007
Clinical & Experimental Immunology
Volume 149, Issue 1, pages 139–145, July 2007
How to Cite
Sasaki, Y., Darmochwal-Kolarz, D., Suzuki, D., Sakai, M., Ito, M., Shima, T., Shiozaki, A., Rolinski, J. and Saito, S. (2007), Proportion of peripheral blood and decidual CD4+ CD25bright regulatory T cells in pre-eclampsia. Clinical & Experimental Immunology, 149: 139–145. doi: 10.1111/j.1365-2249.2007.03397.x
- Issue published online: 25 APR 2007
- Article first published online: 25 APR 2007
- Accepted for publication 5 March 2007
- placental bed biopsy;
- regulatory T cells;
CD4+ CD25bright regulatory T (Treg) cells have been identified as a principle regulator of tolerance during pregnancy. In the setting of pre-eclampsia, however, little is known about the dynamics of these cells. In the current study, we determined CD4+ CD25bright Treg cells in the peripheral blood using flow cytometry and forkhead box P3 (FoxP3+) cells at the placental bed using immunohistochemical staining. Peripheral blood mononuclear cells (PBMC) of 38 pre-eclamptic cases (17 cases Japanese, 21 cases Polish), 40 normal late pregnancy subjects (20 subjects Japanese, 20 subjects Polish), and 21 non-pregnant healthy controls (10 subjects Japanese, 11 subjects Polish) were included. We found the percentage of CD25bright cells within the CD4+ T cell population in PBMC was reduced significantly in both Japanese and Polish pre-eclamptic cases than in normal pregnancy subjects (P < 0·001) and non-pregnant healthy controls (P < 0·001). Also, the percentage of FoxP3+ cells within CD3+ T cells in the placental bed biopsy samples of pre-eclamptic cases were decreased compared to those in normal pregnancy subjects. These findings suggest that a decreased number of Treg cells was present in pre-eclampsia, and these changes might break the maternal tolerance to the fetus.