Present address: Department of Neurology, Klinikum Augsburg, Stenglinstr. 2, 86156 Augsburg, Germany.
Altered innate immune response of plasmacytoid dendritic cells in multiple sclerosis
Article first published online: 29 APR 2009
© 2009 British Society for Immunology
Clinical & Experimental Immunology
Volume 157, Issue 3, pages 332–342, September 2009
How to Cite
Bayas, A., Stasiolek, M., Kruse, N., Toyka, K. V., Selmaj, K. and Gold, R. (2009), Altered innate immune response of plasmacytoid dendritic cells in multiple sclerosis. Clinical & Experimental Immunology, 157: 332–342. doi: 10.1111/j.1365-2249.2009.03964.x
- Issue published online: 27 JUL 2009
- Article first published online: 29 APR 2009
- Accepted for publication 17 April 2009
- dendritic cell;
- multiple sclerosis;
- Toll-like receptor
Plasmacytoid dendritic cells (pDCs) are of crucial importance in immune regulation and response to microbial factors. In multiple sclerosis (MS), pDCs from peripheral blood showed an immature phenotype, but its role in susceptibility to MS is not determined. Because infectious diseases are established triggers of exacerbations in MS, in this study we have characterized the expression of Toll-like receptors (TLR) and the maturation and functional properties of peripheral blood pDCs from clinically stable, untreated MS patients in response to signals of innate immunity. After stimulation of TLR-9, interferon (IFN)-α production by pDCs was significantly lower in MS (n = 12) compared to healthy controls (n = 9). In an allogenic two-step co-culture assay we found an impaired effect of TLR-9 stimulation on IFN-γ expression of autologous naive T cells in MS patients (n = 4). In peripheral blood mononuclear cells, TLR-9 stimulation with type A CpG ODN resulted in a higher expression of TLR-1, -2, -4, -5 and -8 in MS patients (n = 7) compared with healthy controls (n = 11). These findings suggest an altered innate immune response to microbial stimuli in MS patients and may help understanding of why common infectious agents trigger MS attacks.