This work was presented in part at the 16th International Pathogenic Neisseria Conference, 7–12 September, 2008, Rotterdam, the Netherlands, abstract number (P084).
Mannose-binding lectin is present in human semen and modulates cellular adhesion of Neisseria gonorrhoeae in vitro
Article first published online: 9 JUN 2009
© 2009 British Society for Immunology
Clinical & Experimental Immunology
Volume 157, Issue 3, pages 408–414, September 2009
How to Cite
Wing, J. B., Jack, D. L., Lee, M. E., Pacey, A. A., Kinghorn, G. R. and Read, R. C. (2009), Mannose-binding lectin is present in human semen and modulates cellular adhesion of Neisseria gonorrhoeae in vitro. Clinical & Experimental Immunology, 157: 408–414. doi: 10.1111/j.1365-2249.2009.03984.x
- Issue published online: 27 JUL 2009
- Article first published online: 9 JUN 2009
- Accepted for publication 1 June 2009
- mucosal immunity;
- reproductive immunology
Mannose-binding lectin (MBL) is an innate immune molecule present in blood and some mucosal tissues, which can influence microbial attachment and inflammatory responses of host cells during infection. In this study MBL was found to be present at a low concentration in semen samples in the range 1·2–24·9 ng/ml. Co-incubation of bacteria with semen resulted in the binding of MBL to the bacterial surface. Neisseria gonorrhoeae is a common cause of genitourinary infection. MBL bound to N. gonorrhoeae with strain-to-strain variation in the intensity of binding and nature of the bacterial receptor. Pretreatment with MBL concentrations similar to those found in human serum modulated the adhesion of N. gonorrhoeae strain FA1090 but not strain MS11 to epithelial cells. This effect was dose-dependent. This work demonstrates that MBL is present in human semen and modifies cellular responses to N. gonorrhoeae in a concentration-dependent manner.