These authors contributed equally to this work.
Flk-1+ mesenchymal stem cells aggravate collagen-induced arthritis by up-regulating interleukin-6
Article first published online: 4 DEC 2009
© 2009 British Society for Immunology
Clinical & Experimental Immunology
Volume 159, Issue 3, pages 292–302, March 2010
How to Cite
Chen, B., Hu, J., Liao, L., Sun, Z., Han, Q., Song, Z. and Zhao, R. C. (2010), Flk-1+ mesenchymal stem cells aggravate collagen-induced arthritis by up-regulating interleukin-6. Clinical & Experimental Immunology, 159: 292–302. doi: 10.1111/j.1365-2249.2009.04069.x
- Issue published online: 15 JAN 2010
- Article first published online: 4 DEC 2009
- Accepted for publication 3 November 2009
- immune regulation;
- mesenchymal stem cells;
- rheumatoid arthritis
The immunomodulatory ability of mesenchymal stem cells (MSCs) may be used to develop therapies for autoimmune diseases. Flk-1+ MSCs are a population of MSCs with defined phenotype and their safety has been evaluated in Phase 1 clinical trials. We designed this study to evaluate whether Flk-1+ MSCs conferred a therapeutic effect on collagen-induced arthritis (CIA), an animal model of rheumatic arthritis, and to explore the underlying mechanisms. Flk-1+ MSCs, 1–2 × 106, were injected into CIA mice on either day 0 or day 21. The clinical course of arthritis was monitored. Serum cytokine profile was determined by cytometric bead array kit or enzyme-linked immunosorbent assay. Flk-1+ MSCs and splenocytes co-culture was conducted to explore the underlying mechanisms. Flk-1+ MSCs did not confer therapeutic benefits. Clinical symptom scores and histological evaluation suggested aggravation of arthritis in mice treated with MSCs at day 21. Serum cytokine profile analysis showed marked interleukin (IL)-6 secretion immediately after MSC administration. Results of in vitro culture of splenocytes confirmed that the addition of Flk-1+ MSCs promoted splenocyte proliferation and increased IL-6 and IL-17 secretion. Moreover, splenocyte proliferation was also enhanced in mice treated with MSCs at day 21. Accordingly, MSCs at low concentrations were found to promote lipopolysaccharide-primed splenocytes proliferation in an in vitro co-culture system. We propose that Flk-1+ MSCs aggravate arthritis in CIA model by at least up-regulating secretion of IL-6, which favours Th17 differentiation. When Flk-1+ MSCs are used for patients, we should be cautious about subjects with rheumatoid arthritis.