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Fig. S1. Stimulation by a single CD28 monoclonal antibody (mAb) induces proliferation in lamina propria T lymphocytes (LPT) but not peripheral blood T lymphocytes (PBT). CD4+ PBT and LPT were cultured in the presence or absence of two different concentrations of the CD28 mAb 248 [immunoglobulin (Ig)M; 10 and 5 μg/ml] or the CD3 mAb muromonab-CD3 (OKT-3) (0·5 and 0·05 μg/ml) for 72 h. Proliferation was determined by [3H]-thymidine incorporation. Shown are two independent experiments using cells from two different patients. Data are represented as mean ± standard error of the mean of triplicate cultures.

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