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Fig. S1. Dose–response curve of transglutaminase 2 (TG2) induction by tumour necrosis factor (TNF)-α and interferon (IFN)-γ. Human acute monocytic leukaemia cell line (THP-1) cells were incubated for 24 h with different concentrations of TNF-α and/or IFN-γ as indicated. Grey bars correspond to the cytokine concentration selected for further studies; TNF-α (10 ng/ml) and IFN-γ (200 UI/ml).

Fig. S2. Cell viability after cytokine treatments. Cell viability was determined by Trypan blue exclusion in human acute monocytic leukaemia cell line (THP-1) cells treated for 24 h with different concentrations of tumour necrosis factor (TNF)-α and/or interferon (IFN)-γ as indicated. Grey bars correspond to the cytokine concentration selected for further studies; TNF-α (10 ng/ml) and IFN-γ (200 UI/ml).

Fig. S3. Kinetic of transglutaminase 2 (TG2) induction by tumour necrosis factor (TNF)α + interferon (IFN)-γ. Caco-2 and human acute monocytic leukaemia cell line (THP-1) cells were incubated for different times with TNF-α (10 ng/ml) and IFN-γ (200 UI/ml). Levels of TG2 transcript were determined by quantitative real time–polymerase chain reaction (RT–PCR). Results were normalized against β-actin and relative TG2 mRNA levels were referred to the non-stimulated control (value = 1). Data represent means ± standard error of the mean (n = 3). The Mann–Whitney U-test was performed: *P < 0·05; **P < 0·01.

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