Dysregulated immunophenotypic attributes of plasmacytoid but not myeloid dendritic cells in HIV-1 infected individuals in the absence of highly active anti-retroviral therapy
Version of Record online: 5 OCT 2012
© 2012 The Authors. Clinical and Experimental Immunology © 2012 British Society for Immunology
Clinical & Experimental Immunology
Special Issue: Immunological features in clinical autoimmunity: Type 1 diabetes and multiple sclerosis
Volume 170, Issue 2, pages 212–221, November 2012
How to Cite
Benlahrech, A., Yasmin, A., Westrop, S. J., Coleman, A., Herasimtschuk, A., Page, E., Kelleher, P., Gotch, F., Imami, N. and Patterson, S. (2012), Dysregulated immunophenotypic attributes of plasmacytoid but not myeloid dendritic cells in HIV-1 infected individuals in the absence of highly active anti-retroviral therapy. Clinical & Experimental Immunology, 170: 212–221. doi: 10.1111/j.1365-2249.2012.04647.x
- Issue online: 5 OCT 2012
- Version of Record online: 5 OCT 2012
- Accepted manuscript online: 23 JUL 2012 07:05AM EST
- Accepted for publication 5 July 2012
Fig. S1. Six-colour gating strategy for the identification and phenotypic characterization of plasmacytoid dendritic cells (pDC) and myeloid DC (mDC). Peripheral blood mononuclear cells (PBMC) were collected from healthy controls, therapy-naive and highly active anti-retroviral therapy (HAART)-treated patients. Cells were surface-stained with appropriate antibodies and analysed by flow cytometry; 105 live events were collected and identified on the basis of forward- and side-scatter plots (a). Doublets were excluded on the basis of forward-scatter height versus area (b). Total DC were identified on the basis of expression of human leucocyte antigen D-related (HLA-DR) and lack of expression of lineage-associated markers (c). pDC and mDC were then distinguished on the basis of CD123 and CD11c expression, respectively (d). Surface expression of specific markers was determined using appropriate isotype controls (e, f).
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