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Fig. S1. Six-colour gating strategy for the identification and phenotypic characterization of plasmacytoid dendritic cells (pDC) and myeloid DC (mDC). Peripheral blood mononuclear cells (PBMC) were collected from healthy controls, therapy-naive and highly active anti-retroviral therapy (HAART)-treated patients. Cells were surface-stained with appropriate antibodies and analysed by flow cytometry; 105 live events were collected and identified on the basis of forward- and side-scatter plots (a). Doublets were excluded on the basis of forward-scatter height versus area (b). Total DC were identified on the basis of expression of human leucocyte antigen D-related (HLA-DR) and lack of expression of lineage-associated markers (c). pDC and mDC were then distinguished on the basis of CD123 and CD11c expression, respectively (d). Surface expression of specific markers was determined using appropriate isotype controls (e, f).

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