Activated natural killer T cells producing interferon-gamma elicit promoting activity to murine dendritic cell-based autoimmune hepatic inflammation
Article first published online: 1 NOV 2012
© 2012 The Authors Clinical and Experimental Immunology © 2012 British Society for Immunology
Clinical & Experimental Immunology
Special Issue: Immune abnormalities in CVID Tregs and H pylori
Volume 170, Issue 3, pages 274–282, December 2012
How to Cite
Nakano, M., Saeki, C., Takahashi, H., Homma, S., Tajiri, H. and Zeniya, M. (2012), Activated natural killer T cells producing interferon-gamma elicit promoting activity to murine dendritic cell-based autoimmune hepatic inflammation. Clinical & Experimental Immunology, 170: 274–282. doi: 10.1111/j.1365-2249.2012.04664.x
- Issue published online: 1 NOV 2012
- Article first published online: 1 NOV 2012
- Accepted manuscript online: 21 AUG 2012 06:46AM EST
- Manuscript Accepted: 15 AUG 2012
- Health Labor Science Research on Measures
- Ministry of Health, Labor and welfare of Japan
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Fig. S1. The dynamic statistics of natural killer (NK) T cells in the autoimmune hepatic inflammation (AHI) liver and spleen. (A) Absolute number of NK T cells in the liver (a) and spleen (b) in AHI. The number was determined as [total number of mononuclear cells (MNCs) in the liver or spleen] × [the frequency of CD3+NK1·1+ cells] in each group [n = 5, mean ± standard deviation (s.d.), *P < 0·001]. (B) (a) Population of intrahepatic CXCR6+ NK T cells (n = 5, mean ± s.d., *P < 0·001). (b) Expression of CXCL16 in hepatic tissue. Levels of CXCL16 mRNA in each group were determined by quantitative reverse transcription–polymerase chain reaction (qRT–PCR). Bars indicate mean ± s.d., *P < 0·001. (C) (a) Frequency of interferon (IFN)-γ+ NK T cells in each group (n = 5, mean ± s.d., *P < 0·001). (b) Frequency of interleukin (IL)-4+ NK T cells in each group. All experiments were repeated at least three times.
Fig. S2. Frequency of natural killer (NK) T cells in the liver and spleen in autoimmune hepatic inflammation (AHI). Frequencies of NK T cells in intrahepatic major histocompatibility complexes (MHCs) and splenocytes were determined by flow cytometry (n = 5, mean ± standard deviation, *P < 0·001). Experiments were repeated at least three times.
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