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cei4664-sup-0001- fs1.TIF74K

Fig. S1. The dynamic statistics of natural killer (NK) T cells in the autoimmune hepatic inflammation (AHI) liver and spleen. (A) Absolute number of NK T cells in the liver (a) and spleen (b) in AHI. The number was determined as [total number of mononuclear cells (MNCs) in the liver or spleen] × [the frequency of CD3+NK1·1+ cells] in each group [n = 5, mean ± standard deviation (s.d.), *P < 0·001]. (B) (a) Population of intrahepatic CXCR6+ NK T cells (n = 5, mean ± s.d., *P < 0·001). (b) Expression of CXCL16 in hepatic tissue. Levels of CXCL16 mRNA in each group were determined by quantitative reverse transcription–polymerase chain reaction (qRT–PCR). Bars indicate mean ± s.d., *P < 0·001. (C) (a) Frequency of interferon (IFN)-γ+ NK T cells in each group (n = 5, mean ± s.d., *P < 0·001). (b) Frequency of interleukin (IL)-4+ NK T cells in each group. All experiments were repeated at least three times.

cei4664-sup-0002-fs2.TIF65K

Fig. S2. Frequency of natural killer (NK) T cells in the liver and spleen in autoimmune hepatic inflammation (AHI). Frequencies of NK T cells in intrahepatic major histocompatibility complexes (MHCs) and splenocytes were determined by flow cytometry (n = 5, mean ± standard deviation, *P < 0·001). Experiments were repeated at least three times.

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