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Increased levels and adipose tissue expression of visfatin in morbidly obese women: the relationship with pro-inflammatory cytokines

Authors

  • Ximena Terra,

    1. Grup de Recerca en Medicina Aplicada Hospital Joan XXIII, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Tarragona
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  • Teresa Auguet,

    1. Grup de Recerca en Medicina Aplicada Hospital Joan XXIII, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Tarragona
    2. Servei Medicina Interna, Hospital Universitari Joan XXIII, Tarragona
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  • Isabel Quesada,

    1. Grup de Recerca en Medicina Aplicada Hospital Joan XXIII, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Tarragona
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  • Carmen Aguilar,

    1. Grup de Recerca en Medicina Aplicada Hospital Joan XXIII, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Tarragona
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  • Anna Maria Luna,

    1. Grup de Recerca en Medicina Aplicada Hospital Joan XXIII, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Tarragona
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  • Mercé Hernández,

    1. Servei de Cirurgia, Hospital Sant Joan de Reus, Tarragona
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  • Fátima Sabench,

    1. Servei de Cirurgia, Hospital Sant Joan de Reus, Tarragona
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  • José Antonio Porras,

    1. Grup de Recerca en Medicina Aplicada Hospital Joan XXIII, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Tarragona
    2. Servei Medicina Interna, Hospital Universitari Joan XXIII, Tarragona
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  • Salomé Martínez,

    1. Grup de Recerca en Medicina Aplicada Hospital Joan XXIII, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Tarragona
    2. Servei Anatomia Patológica, Hospital Universitari Joan XXIII, Tarragona
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  • Anna Lucas,

    1. Servei d’Endocrinologia i Nutrició, Hospital Germans Tries i Pujol, Badalona, Spain
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  • Silvia Pellitero,

    1. Servei d’Endocrinologia i Nutrició, Hospital Germans Tries i Pujol, Badalona, Spain
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  • Jordi Llutart,

    1. Grup de Recerca en Medicina Aplicada Hospital Joan XXIII, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Tarragona
    2. Servei Medicina Interna, Hospital Universitari Joan XXIII, Tarragona
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  • Daniel del Castillo,

    1. Servei de Cirurgia, Hospital Sant Joan de Reus, Tarragona
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  • Cristóbal Richart

    1. Grup de Recerca en Medicina Aplicada Hospital Joan XXIII, Departament de Medicina i Cirurgia, Universitat Rovira i Virgili (URV), IISPV, Tarragona
    2. Servei Medicina Interna, Hospital Universitari Joan XXIII, Tarragona
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Cristóbal Richart Jurado, Hospital Universitari de Tarragona Joan XXIII, Mallafré Guasch, 4, 43007 Tarragona, Spain. Tel.: +34 977 295 833; Fax: +34 977 295 833; E-mail: crichart.hj23.ics@gencat.cat

Summary

Objective  The controversial results on the physiopathological role of visfatin led us to examine both circulating visfatin levels and gene expression in visceral (VAT) and subcutaneous fat (SAT) in a homogeneous group of morbidly obese women.

Design, patients and measurements  We analysed circulating levels of several adipo/cytokines in 133 Spanish women: 40 lean (C) [body mass index (BMI) < 25 kg/m2] and 93 morbidly obese (MO) (BMI > 40 kg/m2). In the MO group, we found 31 diabetic and 62 nondiabetic subjects. We obtained follow-up blood samples at 6 and 12 months after bariatric surgery from 30 MO patients. We determined the circulating levels of visfatin, adiponectin, interleukin-6 (IL6), C-reactive protein (CRP), resistin and tumour necrosis factor-α (TNFα) by ELISA, and visfatin, adiponectin, IL6, resistin and TNFα gene expression in SAT and VAT by real-time RT-PCR.

Results  Circulating visfatin levels were higher in MO women compared with lean controls (C = 1·43 ± 0·14 μg/l, MO = 3·60 ± 0·29 μg/l, P < 0·001). After bariatric surgery-induced weight loss, visfatin levels were reduced significantly over 12 months. Visfatin expression in SAT and VAT was similar, but significantly higher in MO compared to C and independent of the presence of diabetes mellitus. Circulating visfatin levels were positively related to IL6 and CRP levels. Visfatin gene expression in VAT and SAT was strongly related to IL6 and TNFα expression.

Conclusion  In a homogeneous cohort of morbidly obese women, our findings show that visfatin has a strong relationship with pro-inflammatory factors in severe obesity.

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