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Supporting Information

Additional Supporting Information may be found in the online version of this article under the DOI reference:DOI: 10.1111/j.1365-2311.2010.01256.x

Figure S1. Larval developmental duration (days) of male (a) and female (b) Phaedon cochleariae of the second (s, n, b) and 11th generation (S, N, B) of the three subpopulations and lines (1, 2, 3), respectively, reared on Sinapis alba, Nasturtium officinale, and Brassica rapa. Within the 11th generation, larvae of the Sinapis and Nasturtium lines were either kept on their alternative rearing plant species (S1–S3, N1–N3) or tested on the original host-plant species B. rapa (S1-br–S3-br, N1-br–N3-br). Brassica lines (B1–B3) were continuously kept on B. rapa. White boxes S. alba, dark grey boxes N. officinale, light grey boxes B. rapa as food/test plant. The boxes represent the median, mean (dotted line) and the 25% and 75% percentiles. The whiskers above and below the box indicate the 90th and 10th percentiles; the black dots denote outliers; n = 10–29.

Figure S2. Relative growth rates (day−1) of male (a) and female (b) Phaedon cochleariae of the second (s, n, b) and 11th generation (S, N, B) of the three subpopulations and lines (1–3), respectively, reared on Sinapis alba, Nasturtium officinale, and Brassica rapa. Within the 11th generation, larvae of the Sinapis and Nasturtium lines were either kept on their alternative rearing plant species (S1–S3, N1–N3) or tested on the original host-plant species B. rapa (S1-br–S3-br, N1-br–N3-br). Brassica lines (B1–B3) were continuously kept on B. rapa. For further details see legend of Figure S1.

Figure S3. Pupal body masses (mg) of male (a) and female (b) Phaedon cochleariae of the second (s, n, b) and 11th generation (S, N, B) of the three subpopulations and lines (1–3), respectively, reared on Sinapis alba, Nasturtium officinale, and Brassica rapa. Within the 11th generation larvae of the Sinapis and Nasturtium lines were either kept on their alternative rearing plant species (S1–S3, N1–N3) or tested on the original host-plant species B. rapa (S1-br–S3-br, N1-br–N3-br). Brassica lines (B1–B3) were continuously kept on B. rapa. For further details see legend of Figure S1.

Table S1. General linear mixed model results (t- and P-values) for developmental parameters of the second and 11th generation of Phaedon cochleariae reared on Sinapis alba (s, S1–S3), Nasturtium officinale (n, N1–N3), and Brassica rapa (b, B1–B3). Rearing plant (species offered since the first generation of the experiment), generation, and the interaction of both were included as explanatory variables in the model. Body mass of neonates and line were denoted as random factors. Significant differences (P < 0.05) are highlighted in bold.

Table S2. General linear mixed model results (t- and P-values) for performance of Phaedon cochleariae on Brassica rapa in the 11th generation after different selection regimes. Beetles were reared either on Sinapis alba, Nasturtium officinale, or on B. rapa for 10 generations and tested on the original host B. rapa in the 11th generation (S1br–S3br, N1br–N3br, B1–B3; see Fig. 1). Rearing plant species (S. alba, N. officinale, or B. rapa), was used as fixed effect. Body mass of neonates and line were denoted as random factors. Significant differences (P < 0.05) are highlighted in bold.

Table S3. Feeding preferences of male and female Phaedon cochleariae of the second (three subpopulations s, n, b) and 11th generation (lines 1–3 of Sinapis, Nasturtium, Brassica), respectively. Leaf discs of the rearing plant species and the original plant species (for Sinapis and Nasturtium subpopulations and lines) or of the alternative host-plant species (for the Brassica subpopulation and lines), respectively, were offered in dual choice tests. Mean feeding amounts (ng dry weight) ± SD on leaf discs of both offered plant species are shown. Preference indices (PI) were calculated as PI = (feeding amount on rearing plant species – feeding amount on original or alternative plant species)/(total amount consumed); PI = 0 indicates no preference, PI > 0 indicates preference for rearing plant. Number of replicates (n) is given in parentheses.

Table S4. Oviposition preferences of Phaedon cochleariae females of the 11th generation of the lines 1–3 of the Sinapis, Nasturtium and Brassica populations. Leaf discs of the rearing plant species and the original plant species (for Sinapis and Nasturtium lines) or of the alternative host-plant species (for the Brassica lines), respectively, were offered in dual choice tests. Mean egg number per female and plant species was calculated as eggs laid within 24 h averaged over five consecutive days. Preference indices (PI) were calculated as PI = (number of eggs on rearing plant species – number of eggs on original or alternative plant species)/(total number of eggs); PI = 0 indicates no preference, PI > 0 indicates preference for rearing plant. Number of replicates (n) is given in parenthesis following PI.

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