The test was developed with 13 strains of C. michiganensis
from different geographical origin 11 from Australia, one from the US one from the United Kingdom. It also included C. michiganensis
, C. michiganensis
subsp. michiganensis, Arthrobacter ilicis, Curtobacterium flaccumfaciens
, Rathayibacter iranicus
, R. rathayi
, R. tritici
and R. toxicus,
and Corynebacterium agropyri.
Other bacteria occurring naturally in and around lucerne plants were also included. The average minimum number of cells detected by the test when performed on pure cultures was 3.4 (mean values of three replicates) cells per PCR reaction. Similar sensitivity was achieved for plant extracts spiked with C. michiganensis
and incubated for 2 h. In comparative studies this test was shown to be more sensitive than the test of Bach et al. (2003)
described in part B.