Laboratory for Experimental Immunology (M. Langeveld, L. E. Gamadia), Renal Transplant Unit (L. E. Gamadia, i. J. M. ten Berge), Department of Internal Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands.
T-lymphocyte subset distribution in human spleen
Article first published online: 24 MAR 2006
European Journal of Clinical Investigation
Volume 36, Issue 4, pages 250–256, April 2006
How to Cite
Langeveld, M., Gamadia, L. E. and Ten Berge, I. J. M. (2006), T-lymphocyte subset distribution in human spleen. European Journal of Clinical Investigation, 36: 250–256. doi: 10.1111/j.1365-2362.2006.01626.x
- Issue published online: 24 MAR 2006
- Article first published online: 24 MAR 2006
- Received 14 January 2005; accepted 1 February 2006
- T cells;
Background When analyzing human cellular immune responses, most focus is placed on the peripheral blood (PB) and, to a lesser extent, the lymph nodes. To date the spleen has not been analyzed with regard to its role in adaptive cellular immunity and more notably not with respect to T-cell immune responses.
Materials and methods We analyzed the splenic lymphocyte compartment in comparison with the PB lymphocyte compartment regarding the number of NK cells, B cells, CD4+, CD8+ T cells and CMV-specific CD8+ T cells. Furthermore, we analyzed the distribution of naive, memory and effector subsets of CD4+ and CD8+ T cells in these compartments.
Results The spleen contains proportionally more B cells and less CD4+ and CD8+ T cells than PB. The percentage of CD8+ T cells is greater in the spleen, leading to an inverse CD4/CD8 ratio. Both splenic CD4+ and CD8+ T-cell populations show a greater number of activated cells, and splenic CD8+ T cells show a more differentiated cytotoxic CD27−CD45RA+ memory phenotype.
Conclusions Our findings show that the distribution of the different lymphocyte subsets is markedly different between the spleen and the PB, thus inferring an important and distinct role for the spleen in CD4+ and CD8+ T-cell activation.