Department of Internal Medicine, Division of Cardiology, Helsinki University Central Hospital, Helsinki, Finland (M. Granér, J. Kahri, M. S. Nieminen, M. Syvänne, M. R. Taskinen); JIMRO Co Ltd, Takasaki, Gunma, Japan (T. Nakano); Department of Public Health, University of Helsinki, Finland (S. J. Sarna).
Impact of postprandial lipaemia on low-density lipoprotein (LDL) size and oxidized LDL in patients with coronary artery disease
Article first published online: 9 OCT 2006
European Journal of Clinical Investigation
Volume 36, Issue 11, pages 764–770, November 2006
How to Cite
Granér, M., Kahri, J., Nakano, T., Sarna, S. J., Nieminen, M. S., Syvänne, M. and Taskinen, M. R. (2006), Impact of postprandial lipaemia on low-density lipoprotein (LDL) size and oxidized LDL in patients with coronary artery disease. European Journal of Clinical Investigation, 36: 764–770. doi: 10.1111/j.1365-2362.2006.01720.x
- Issue published online: 9 OCT 2006
- Article first published online: 9 OCT 2006
- Received 23 April 2006; accepted 24.July 2006
- Coronary artery disease;
- oxidized LDL;
- quantitative coronary angiography;
- remnant lipoprotein particles;
- triglyceride-rich lipoproteins
Background Remnant lipoprotein particles (RLPs) and oxidative stress are components of postprandial state. We investigated the concentrations of triglyceride-rich lipoproteins (TRLs), RLPs, low-density lipoprotein (LDL) size, and oxidized LDL (oxLDL) during alimentary lipaemia, and evaluated whether changes among these variables could be associated with the severity and extent of coronary artery disease (CAD).
Materials and methods Eighty men and 27 women with clinically suspected CAD underwent quantitative coronary angiography (QCA). TRLs were isolated by density gradient ultracentrifugation before and 6 h after an oral fat load. RLPs were measured by an immunoseparation method, oxLDL by ELISA, and LDL size by gradient gel electrophoresis.
Results Triglycerides, apolipoprotein (apo) B-48, and apoB-100 concentration in Swedberg flotation units (Sf) > 400 and in Sf 12–400 fractions were markedly increased at 6 h. Postprandial cholesterol content of RLPs (RLP-C) correlated with respective triglycerides in Sf > 400 (r = 0·737) and Sf 12–400 (r = 0·857), apoB-48 in Sf > 400 (r = 0·710) and Sf 12–400 (r = 0·664), apoB-100 in Sf > 400 (r = 0·812) and Sf 12–400 (r = 0·533). RLP-C correlated with oxLDL both in fasting and in fed state (r = 0·482 and r = 0·543, respectively) and inversely with LDL size (r = –0·459 and r = −0·442, respectively). (P < 0·001 for all). OxLDL was elevated postprandially (P < 0·001). In multivariate analysis, oxLDL was a determinant of severity and extent of CAD.
Conclusion Postprandial state is associated with oxidative stress. The magnitude of oxLDL increases during alimentary lipaemia and is associated with coronary atherosclerosis.