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High extracellular induced sputum haem oxygenase-1 in sarcoidosis and chronic beryllium disease
Article first published online: 27 APR 2009
DOI: 10.1111/j.1365-2362.2009.02137.x
© 2009 The Authors. Journal Compilation © 2009 Stichting European Society for Clinical Investigation Journal Foundation
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How to Cite
Kokturk, N., Sabag, M., Stark, M., Grief, J. and Fireman, E. (2009), High extracellular induced sputum haem oxygenase-1 in sarcoidosis and chronic beryllium disease. European Journal of Clinical Investigation, 39: 584–590. doi: 10.1111/j.1365-2362.2009.02137.x
Publication History
- Issue published online: 5 JUN 2009
- Article first published online: 27 APR 2009
- Received 20 August 2008; accepted 5 March 2009
- Abstract
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Keywords:
- Heme oxygenase one;
- induced sputum;
- sarcoidosis
Abstract
Background Sarcoidosis and chronic beryllium disease (CBD) are inflammatory conditions in which oxidative stress state may be crucial for disease outcome. This study compares haem oxygenase-1 (HO-1) extracellular activity for the first time in patients with sarcoidosis or CBD and in healthy controls.
Materials and methods Induced sputum was recovered using a standard protocol. Pulmonary function tests (PFT) were performed by conventional methods. T lymphocyte subsets (CD4 and CD8) were measured by flow activated cell shorter (FACS). The HO-1 and nitrite levels were measured by a bilirubin-biliverdin reductase-dependant reaction and Greiss reaction respectively. Ferritin and iron levels were measured by enzymatic reaction and chemiluminometric immunoassay respectively.
Results The mean percentage of lymphocytes was significantly higher in the 36 sarcoid patients compared with that in the 17 controls (P = 0·001). The mean CD4/CD8 ratio was significantly higher in the sarcoid and the 10 CBD patients compared to that in controls (P = 0·000 and 0·002 respectively), as was the mean HO-1 activity (P = 0·045 and 0·041 respectively). The HO-1 activity did not differ with the sarcoidosis stage. The HO-1 level and PFT parameters were negatively correlated. The differences in mean nitrite, ferritin and iron levels were non-significant between the three groups. The HO-1 and ferritin levels were correlated (P = 0·008).
Conclusions We succeeded in non-invasively measuring the activity of HO-1 from cells of airways in spite of its being an intracellular enzyme. The HO-1 levels in sarcoidosis and CBD were abnormally elevated.