Presented in part: Digestive Disease Week 2010, New Orleans, USA, May 2010 and United European Gastroenterology Week 2010, Barcelona, Spain, October 2010.
Predictors of indeterminate IFN-γ release assay in screening for latent TB in inflammatory bowel diseases
Version of Record online: 17 MAR 2011
© 2011 The Authors. European Journal of Clinical Investigation © 2011 Stichting European Society for Clinical Investigation Journal Foundation
European Journal of Clinical Investigation
Volume 41, Issue 10, pages 1071–1076, October 2011
How to Cite
Papay, P., Eser, A., Winkler, S., Frantal, S., Primas, C., Miehsler, W., Angelberger, S., Novacek, G., Mikulits, A., Vogelsang, H. and Reinisch, W. (2011), Predictors of indeterminate IFN-γ release assay in screening for latent TB in inflammatory bowel diseases. European Journal of Clinical Investigation, 41: 1071–1076. doi: 10.1111/j.1365-2362.2011.02502.x
- Issue online: 9 SEP 2011
- Version of Record online: 17 MAR 2011
- Received 5 November 2010; accepted 7 February 2011
- Inflammatory bowel disease;
- interferon-gamma release assay;
- latent tuberculosis
Eur J Clin Invest 2011; 41 (10): 1071–1076
Background IFN-γ release assays (IGRA), widely used for latent tuberculosis screening prior to anti-TNF-α treatment, are limited by indeterminate results in patients under immunomodulatory (IM) therapy. The aim of our observational study was to delineate factors associated with indeterminate IGRA results.
Methods A total of 190 patients with inflammatory bowel disease were included. IGRA was indeterminate if the result of IFN-γ concentration was < 0·35 IU mL−1 for tuberculosis-specific antigens and < 0·5 IU mL−1 for the positive control. Predictors for indeterminate results were delineated from multivariate logistic regression.
Results IFN-γ release assays was indeterminate in 26/190 (13·7%) patients. Indeterminate IGRA were associated with lower serum albumin levels (odds ratio [OR] 0·88, 95% confidence interval [CI] 0·79–0·96), lower absolute lymphocyte count (OR 0·39, 95% CI 0·18–0·75) and double IM therapy (OR 2·98, 95% CI 0·95–8·90). Sub-analysis of IM therapy revealed an association of steroid therapy with indeterminate IGRA (OR 3·19, 95% CI 1·35–7·70). Hypoalbuminaemia increased the risk of indeterminate IGRA by (OR 2·97, 95% CI 1·03–8·61) and lymphopaenia by (OR 3·28, 95% CI 1·41–7·65). After a mean of 18·5 ± 14·4 days, retesting of IGRA in 18 patients with indeterminate results yielded 9 negative vs. 9 indeterminate results.
Conclusions Our results reveal associations of indeterminate IGRA with low serum albumin levels and absolute lymphocyte count and double IM therapy. IGRA testing appears best to be performed prior to initiation of IM therapy in patients with inflammatory bowel disease.