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Plasma proteomic analysis in obese and overweight prepubertal children

Authors

  • Zoi Galata,

    1. Division of Endocrinology and Metabolism, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
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  • George Moschonis,

    1. Department of Nutrition and Dietetics, Harokopio University of Athens, Athens, Greece
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  • Manousos Makridakis,

    1. Division of Biotechnology, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
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  • Ploumisti Dimitraki,

    1. Division of Biotechnology, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
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  • Nicolas C. Nicolaides,

    1. Division of Endocrinology and Metabolism, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
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  • Yannis Manios,

    1. Department of Nutrition and Dietetics, Harokopio University of Athens, Athens, Greece
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  • Anastasia Bartzeliotou,

    1. Department of Clinical Biochemistry, University of Athens Medical School, ‘Aghia Sophia’ Children’s Hospital, Athens, Greece
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  • George P. Chrousos,

    1. Division of Endocrinology and Metabolism, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
    2. First Department of Pediatrics, University of Athens Medical School, ‘Aghia Sophia’ Children’s Hospital, Athens, Greece
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  • Evangelia Charmandari

    1. Division of Endocrinology and Metabolism, Biomedical Research Foundation of the Academy of Athens, Athens, Greece
    2. First Department of Pediatrics, University of Athens Medical School, ‘Aghia Sophia’ Children’s Hospital, Athens, Greece
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Evangelia Charmandari, MD, Division of Endocrinology and Metabolism, Biomedical Research Foundation of the Academy of Athens, 4 Soranou tou Efessiou Street, Athens 11527, Greece. Tel.: +30 210 659 7546; fax: +30 210 659 7545; e-mail: evangelia.charmandari@googlemail.com

Abstract

Eur J Clin Invest 2011; 41 (12): 1275–1283

Abstract

Background  Childhood obesity represents one of the most challenging health problems of our century and is associated with significant morbidity and mortality in adult life. Proteomics is a large-scale analysis of proteins, which provides, information on protein expression levels, post-translational modifications, subcellular localization and interactions.

Objective  To investigate whether obesity in childhood is associated with alterations in plasma protein expression profiles.

Methods  Plasma samples from 10 obese [age: 10·75 ± 0·16 year; body mass index (BMI): 27·50 ± 0·69 kg m−2], 10 overweight (age: 10·54 ± 0·1 year; BMI: 21·88 ± 0·28 kg m−2) and 10 normal-weight (age: 10·89 ± 0·19 year; BMI: 18·34 ± 0·42kg m−2) prepubertal boys were subjected to protein fractionation and analysed by two-dimensional electrophoresis, followed by protein identification using matrix-assisted laser desorption time-of-flight mass spectrometry. Fasting plasma glucose and serum insulin, lipid and apolipopoprotein concentrations were determined in all subjects.

Results  The expression of apolipoprotein (Apo) A-I (ApoA-I) was significantly lower in obese and overweight children compared with children of normal BMI (< 0·05). The expression of ApoE was significantly lower in overweight compared with normal-weight children (< 0·05), while that of ApoA-IV was significantly higher in obese children compared with their normal counterparts (< 0·01). Serum ApoA-I concentrations were significantly lower in obese (147 ± 4·27mg dL−1) and overweight (145·5 ± 9·65mg dL−1) than in normal-weight (157 ± 8·77mg dL−1; = 0·036) children.

Conclusions  Obese and overweight prepubertal children demonstrated prominent alterations in the expression of plasma apolipoproteins compared with their normal counterparts. Low ApoA-I plasma expression levels and serum concentrations in obesity might be present in childhood before any significant alterations in total or high-density lipoprotein-cholesterol concentrations are documented. We recommend that serum ApoA-I concentrations are determined in all overweight and obese children.

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