Determinants of plasma vitamin D levels in patients with acute coronary syndromes
Article first published online: 25 MAY 2011
© 2011 The Authors. European Journal of Clinical Investigation © 2011 Stichting European Society for Clinical Investigation Journal Foundation
European Journal of Clinical Investigation
Volume 41, Issue 12, pages 1299–1309, December 2011
How to Cite
Rodriguez, G., Starr, A. Z., Czernuszewicz, G. Z., Manhas, A., Alhariri, A., Willerson, J. T., Reist, C. J., Pieper, K., Mahaffey, K. W., Marian, A. J. and Kleiman, N. S. (2011), Determinants of plasma vitamin D levels in patients with acute coronary syndromes. European Journal of Clinical Investigation, 41: 1299–1309. doi: 10.1111/j.1365-2362.2011.02540.x
- Issue published online: 16 NOV 2011
- Article first published online: 25 MAY 2011
- Received 26 November 2010; accepted 14 April 2011
- Acute coronary syndromes;
- vitamin D
Eur J Clin Invest 2011; 41 (12): 1299–1309
Background Vitamin D is implicated in various biological functions ranging from cellular proliferation to immunity. Vitamin D deficiency is associated with an increased risk of several diseases including coronary atherosclerosis.
Materials and methods We measured plasma 25(OH)D3 level in 224 patients with acute coronary syndromes (ACS) and 209 control individuals by ELISA. We genotyped the study populations for 11 single nucleotide polymorphisms (SNPs) in seven genes involved in vitamin D biosynthesis and metabolism by 5′ nuclease assays.
Results The mean and median plasma 25(OH)D3 levels were not significantly different between patients with ACS and controls (median: 22·06 vs. 22·24 ng mL−1, respectively, P = 0·618). Plasma 25(OH)D3 level was < 20 ng mL−1 in 175/433 (40%) and < 30 ng mL−1 in 333/433 (77%) participants. Only four individuals had plasma 25(OH)D3 levels of above 60 ng mL−1. African-American and Hispanic populations, women and those with diabetes mellitus had significantly lower plasma 25(OH)D3 levels. In multivariable regression analysis, age, sex, diabetes mellitus, body weight, rs2762933 (CYP24A1) and rs6055987 (PLCB1) SNPs were independent predictors of plasma 25(OH)D3 level in the Caucasian population.
Conclusions We found no difference in mean plasma vitamin D levels between patients with ACS and controls. Differences in population characteristics between the two study groups including medications use and the lack of data on vitamin D, calcium and multivitamin supplements intake as well as the relatively small sample size of the populations could confound the results. Ethnic background, sex, age, body weight and SNPs in CYP24A1 and PLCB1 were independent determinants of plasma vitamin D levels.