Profiling the expression of interleukin (IL)-28 and IL-28 receptor α in systemic lupus erythematosus patients
Article first published online: 27 JUN 2011
© 2011 The Authors. European Journal of Clinical Investigation © 2011 Stichting European Society for Clinical Investigation Journal Foundation
European Journal of Clinical Investigation
Volume 42, Issue 1, pages 61–69, January 2012
How to Cite
Lin, S.-C., Kuo, C.-C., Tsao, J.-T. and Lin, L.-J. (2012), Profiling the expression of interleukin (IL)-28 and IL-28 receptor α in systemic lupus erythematosus patients. European Journal of Clinical Investigation, 42: 61–69. doi: 10.1111/j.1365-2362.2011.02557.x
- Issue published online: 7 DEC 2011
- Article first published online: 27 JUN 2011
- Accepted manuscript online: 21 MAY 2011 08:14AM EST
- Received 6 October 2010; accepted 18 May 2011
- interleukin (IL)-28;
- systemic lupus erythematosus
Eur J Clin Invest 2012; 42 (1): 61–69
Background Interleukin (IL)-28 is an interferon-λ-family member involved in immunity against viral infection and tumour. We here determined the expression profiles of IL-28 and IL-28 receptor α (IL-28RA) in patients with systemic lupus erythematosus (SLE) to evaluate the possibility that IL-28 is linked to the pathogenesis of SLE.
Materials and methods The serum IL-28 protein levels were determined by ELISA, and the IL-28 and IL-28RA transcript levels in peripheral blood mononuclear cells (PBMCs) and peripheral blood T cells were determined by RT-PCR. The levels in patients with SLE with the active disease activity were statistically compared with those in normal controls.
Results IL-28 protein in sera and IL-28 transcripts in PBMCs and unactivated T cells were detectable only in some individuals, and IL-28 transcripts in T cells were induced by cell activation with anti-CD2, anti-CD3 and anti-CD28 antibodies. However, compared with normal controls, patients with SLE more frequently had detectable IL-28 protein in serum and had the higher IL-28 transcript levels in activated CD4+ T cells, but not activated CD8+ T cells. Two IL-28RA transcripts isoforms were detected in PBMCs and T cells, and their levels in patients with SLE were comparable with those in normal controls.
Conclusions The expression of IL-28, a T-cell autocrine factor, is dysregulated in patients with SLE, supporting the possibility that IL-28 may contribute to some of the SLE pathogenesis.