Analysis, purification and quantification of extracellular DNA from aquatic environments
Article first published online: 29 MAY 2006
Volume 25, Issue 3, pages 525–532, June 1991
How to Cite
BEEBEE, T. J. C. (1991), Analysis, purification and quantification of extracellular DNA from aquatic environments. Freshwater Biology, 25: 525–532. doi: 10.1111/j.1365-2427.1991.tb01395.x
- Issue published online: 29 MAY 2006
- Article first published online: 29 MAY 2006
- (Manuscript accepted 4 January 1991)
SUMMARY 1. The presence of extracellular DNA, but not RNA, at μg 1−1 concentrations was demonstrated in seawater, river water and in a series of eutrophic lowland ponds.
2. Extracellular DNA in natural waters invariably comprised two major components: one of high molecular weight (>20 kb) derived from viruses, and another of low molecular weight (1–500 bp) and apparently free in solution. Similar species of DNA molecules were produced within 2 weeks in simple laboratory modules.
3. A purification procedure was developed which separated extracellular DNA from other components of dissolved organic matter, and which also separated viral from soluble DNA. Both types of DNA were purified sufficiently to constitute active templates for enzymes used in DNA manipulation.