Alarm cues experienced by cane toad tadpoles affect post-metamorphic morphology and chemical defences

Authors


*Correspondence author. E-mail: rics@bio.usyd.edu.au

Summary

  • 1In many anuran species, larvae modify their developmental trajectories and behaviour in response to chemical cues that predict predator risk. Recent reviews highlight a dearth of studies on delayed (post-metamorphic) consequences of larval experience.
  • 2We raised cane toad (Bufo marinus) tadpoles either under control conditions or in the presence of non-lethal predator cues (crushed conspecifics).
  • 3Exposure to these chemical cues massively reduced size at metamorphosis, as predicted by theory. Parotoid glands were larger relative to body size in post-metamorphic animals from the experimental treatment, suggesting higher investment in chemical defences.
  • 4Exposure to chemical cues from crushed conspecifics during larval life reduced total bufadienolide content of metamorphs, but increased amounts of one specific bufadienolide (bufalin).
  • 5Hence, cane toads respond to perceived predation risk in the aquatic environment by metamorphosing at a smaller size and modifying their investment in defensive toxins during post-metamorphic life.
  • 6Phenotypically flexible responses to larval conditions vary among amphibian taxa, and can involve significant carry-over effects into post-metamorphic life.

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