New model for assembly dynamics of bacterial tubulin in relation to the stages of DNA replication

Authors

  • Ippei Inoue,

    1. National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka 411-8540, Japan
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    • a

      Present address: Institute of Life Sciences, Ajinomoto Co., Inc., 1-1, Suzuki-cho, Kawasaki-ku, Kawasaki-shi, Kanagawa 210-8681, Japan

  • Reiko Ino,

    1. Flow Cytometry, Life Science Marketing, Beckman Coulter K.K., TOC Ariake West Tower, 2-5-7, Ariake, Koto-ku, Tokyo 135-0063, Japan
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  • Akiko Nishimura

    Corresponding author
    1. National Institute of Genetics, Research Organization of Information and Systems, Mishima, Shizuoka 411-8540, Japan
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    • b

      Present address: Research Institute for Biological Function, Chubu University, 1200 Matsumoto, Kasugai-shi, Aichi-ken 487-8501, Japan


  • Communicated by: Hiroji Aiba

* Correspondence: anishimu@w2.dion.ne.jp

Abstract

How living cells receive their genome through cell division has been one of the important questions of biology. In prokaryotes, cell division starts with formation of a ring-shaped microtubule-like structure, FtsZ-ring, at the potential division site. All the previous models suggested that FtsZ-ring is formed coupling to termination or far after initiation of DNA replication. In contrast, we demonstrated that a close communication with DNA replication is maintained throughout the cell cycle. FtsZ starts to assemble to the cell center coupling to initiation of DNA replication, and stabilizes as FtsZ-ring at its termination, but does not constrict before separation of nucleoids. This combination of a positive and a negative control would guarantee that a successful replication event would inevitably induce one cell division such that each of the daughter cells would receive one and only one daughter nucleoid.

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