These two authors contributed equally to this work.
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Ctf4 coordinates the progression of helicase and DNA polymerase α
Article first published online: 3 JUN 2009
DOI: 10.1111/j.1365-2443.2009.01310.x
© 2009 The Authors. Journal compilation © 2009 by the Molecular Biology Society of Japan/Blackwell Publishing Ltd
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How to Cite
Tanaka, H., Katou, Y., Yagura, M., Saitoh, K., Itoh, T., Araki, H., Bando, M. and Shirahige, K. (2009), Ctf4 coordinates the progression of helicase and DNA polymerase α. Genes to Cells, 14: 807–820. doi: 10.1111/j.1365-2443.2009.01310.x
Publication History
- Issue published online: 24 JUN 2009
- Article first published online: 3 JUN 2009
- Received: 30 January 2009Accepted: 6 April 2009
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Ctf4 is a protein conserved in eukaryotes and a constituent of the replisome progression complex. It also plays a role in the establishment of sister chromatid cohesion. In our current study, we demonstrate that the replication checkpoint is activated in the absence of Ctf4, and that the interaction between the MCM helicase-go ichi ni san (GINS) complex and DNA polymerase α (Pol α)-primase is destabilized specifically in a ctf4Δ mutant. An in vitro interaction between GINS and DNA Pol α was also found to be mediated by Ctf4. The same interaction was not affected in the absence of the replication checkpoint mediators Tof1 or Mrc1. In ctf4Δ cells, DNA pol α became significantly unstable and was barely detectable at the replication forks in HU. In contrast, the quantities of helicase and DNA pol ɛ bound to replication forks were almost unchanged but their localizations were widely and abnormally dispersed in the mutant cells compared with wild type. These results lead us to propose that Ctf4 is a key connector between DNA helicase and Pol α and is required for the coordinated progression of the replisome.