Neural crest-derived multipotent cells in the adult mouse iris stroma

Authors

  • Miki Kikuchi,

    1. Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan
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  • Ryuhei Hayashi,

    1. Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan
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  • Sachiko Kanakubo,

    1. Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan
    2. Division of Developmental Neuroscience, Center for Translational and Advanced Animal Research, Tohoku University School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai 980-8574, Japan
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  • Ayumi Ogasawara,

    1. Division of Developmental Neuroscience, Center for Translational and Advanced Animal Research, Tohoku University School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai 980-8574, Japan
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  • Masayuki Yamato,

    1. Institute of Advanced Biomedical Engineering and Science, Tokyo Women’s Medical University, Tokyo, Japan
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  • Noriko Osumi,

    1. Division of Developmental Neuroscience, Center for Translational and Advanced Animal Research, Tohoku University School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai 980-8574, Japan
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  • Kohji Nishida

    Corresponding author
    1. Department of Ophthalmology, Tohoku University School of Medicine, Sendai, Japan
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  • Communicated by: Masayuki Yamamoto (Tohoku University)

knishida@oph.med.tohoku.ac.jp

Abstract

The purpose of this study was to characterize neural crest–derived cells within the adult murine iris. The iris was isolated from P0-Cre/Floxed-EGFP transgenic (TG) mice. The isolated iris cells formed EGFP-positive spheres on non-adhesive culture plates. Immunostaining showed that these EGFP-positive spheres expressed neural crest markers including Sox10 and p75NTR, and these cells showing in vitro sphere-forming ability were originally resided in the iris stroma (IS), in vivo. Real-time RT-PCR showed that the EGFP-positive spheres expressed significantly higher levels of the neural crest markers than EGFP-negative spheres and bone marrow–derived mesenchymal stem cells. Furthermore, the iris stromal sphere had capability to differentiate into various cell lineages including smooth muscle and cartilage. These data indicate that neural crest–derived multipotent cells can be isolated from the murine IS and expanded in sphere culture.

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