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Appendix S1. Temporal development of hypolimnetic water temperatures as a function of stratification depth (circles=2 m, triangles=4 m, squares=8 m) and temperature treatment. Gray lines and symbols=‘ambient’; black lines and symbols=‘cold’ temperature. Hypolimnetic temperatures were integrated from the depth of stratification to the bottom of the water column at 10 m.

Appendix S2. Temporal development of the epilimnetic densities of (a–c) ciliates, (d–f) phytoplankton, and (g–i) Daphniahyalina as a function of stratification depth (2, 4, 8 m) and temperature treatment. Gray lines and open circles=‘ambient’, black lines and filled circles=‘cold’ temperature. Solid and broken lines mark the two replicates in each treatment.

Appendix S3. Temporal development of hypolimnetic densities of (a–c) phytoplankton and (d–f) Daphnia as a function of stratification depth (2, 4, 8 m) and temperature treatment (gray lines and open circles=‘ambient’; black lines and filled circles=‘cold’ temperature). Solid and broken lines mark the two replicates in each treatment.

Appendix S4. Temporal development and timing of cardinal events in the seasonal succession of (a–d) total phytoplankton biomass and (e–h) total Daphnia abundance (integrated over the entire water column, i.e. epi- plus hypolimnion) as a function of stratification depth (2, 4, 8 m) and temperature treatment. Gray lines and open circles=‘ambient’; black lines and filled circles=‘cold’ temperature. Solid and broken lines mark the two replicates in each treatment. White bars and gray diamonds=‘ambient’; gray bars and black diamonds=‘cold’ temperature. Diamonds mark the timing of population peaks. The lower end of the bar marks the onset of population development (±1 SE) and for phytoplankton the upper end of the bar marks the end of population decline (±1 SE). The timing of the end of the Daphnia decline is not shown, because densities were above the required threshold values in most treatments. Note that, with respect to timing, depth-integrated dynamics of phytoplankton and Daphnia in the entire water column followed closely the corresponding epilimnetic dynamics (compare Appendix S3 d, h with Figs. 2 b, c). This is indicated by close correlations between the timing of all cardinal events (onset, peak and end of phytoplankton bloom, onset and peak of Daphnia bloom) in the epilimnion and in the entire water column (all Pearson’s r≥0.76, p≤0.004; n=12, except for the onset of the Daphnia peak in the entire water column, where the ‘cold’ 2 m treatments were excluded as outliers and n=10).

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FilenameFormatSizeDescription
GCB_2134_sm_appendix-s1.pdf10KSupporting info item
GCB_2134_sm_appendix-s2.pdf42KSupporting info item
GCB_2134_sm_appendix-s3.pdf28KSupporting info item
GCB_2134_sm_appendix-s4.pdf34KSupporting info item

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.