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gcb2575-sup-0001-FigureS1_AA.tifimage/tif757KFigure S1. Steady-state concentrations of nutrients in cultures and total nitrogen and phosphorous consumed. Nutrients were measured in the four reactors with steady-state densities of Emiliania huxleyi at 500 000 cell mL−1. Nitrogen (N) and phosphate consumed were derived as the difference between the steady-state concentrations in the cyclostats and that in the inflow-media. Low CO2 and NH4+ + NO3 (LCO2 NH4+ + NO3, dark gray bars), low CO2 and NO3 (LCO2 NO3, light gray bars), high CO2 and NH4+ + NO3 (HCO2 NH4++NO3, black bars) and high CO2 and NO3 (HCO2 NO3, white bars). Data are mean ± SE of at least four samples taken every third day for each culture condition.
gcb2575-sup-0002-FigureS2_AA.tifimage/tif789KFigure S2. pH and pCO2 diurnal variation in Emiliania huxleyi cultures.
gcb2575-sup-0003-FigureS3_AA.tifimage/tif807KFigure S3. Growth curves of Emiliania huxleyi cultures on different NH4+ concentrations.
gcb2575-sup-0004-FigureS4_AA.tifimage/tif3367KFigure S4. Effects of N source and pCO2 levels on coccolithophore morphology. Scanning electron microscope (SEM) images of Emiliania huxleyi sampled 14 h into the light period. Cultures were grown at low CO2 with NH4+ + NO3 (a) or NO3 (b) as N source, and high CO2 with NH4+ + NO3 (c) or NO3 (d) as N source. Scale bar in overview images 10 μm, scale bar in detail images 2 μm.
gcb2575-sup-0005-FigureS5_AA.tifimage/tif24657KFigure S5. Instant calcification rates and primary production rates of Emiliania huxleyi cultures. Measurements were determined using radio-labeled H14CO3, 4 and 12 hours into the photoperiod (10 am and 6 pm, a and b respectively) for each growth condition. Low CO2 with NH4+ + NO3 (LCO2 NH4+ + NO3), low CO2 with NO3 (LCO2 NO3), high CO2 with NH4+ + NO3 (HCO2 NH4+ + NO3), high CO2 and NO3 (HCO2 NO3). Bar chart data are given as mean ± SE for at least three replicates taken every third day for each culture condition. Pie charts are given as percentages.
gcb2575-sup-0006-FigureS6_AA.tifimage/tif24656KFigure S6. Correlation between growth rates and particulate carbon content. PIC, POC and TPC were determined at 14 h into the light phase (2 h before the dark phase). PIC (blue circles), POC (red squares) and TPC (green triangles). Corresponding linear regression equations and R2 are: y = −5.2x + 13.6, R2 = 0.25; y = 11.1x + 1, R2 = 0.76 and y = 5.89x + 14.6, R2 = 0.1625, respectively.
gcb2575-sup-0007-FigureS7_AA.tifimage/tif24656KFigure S7. Emiliania huxleyi cell volume for each growth condition. Cell volumes were determined by flow cytometry, four hours after the dark onset (2 am) and four, eight, 12 and 14 h after the light onset (10 am, 2 pm, 6 pm, 8 pm, respectively) for each growth condition. LCO2 NH4+ + NO3 (dark gray bars), LCO2 NO3 (light gray bars), HCO2 NH4+ + NO3 (black bars), HCO2 NO3 (white bars). Data are mean ± SE, the number of samples (n, taken every third day for each culture condition) is 6, 2, 7, 2, 6 at 2 am, 10 am, 2 pm, 6 pm and 8 pm, respectively.
gcb2575-sup-0008-TableS1.docWord document73KTable S1. Growth rates and maximum PSII photosynthetic efficiencies of Emiliania huxleyi cultures on different NH4+ concentrations.
gcb2575-sup-0009-TableS2.docWord document69KTable S2.  pCO2 and N source interaction.

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