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gcb2686-sup-0001-FigS1.pdfapplication/PDF81KFigure S1. Interactive effect between elevated temperature and nitrogen addition. The lipid biomass (nmol lipid g dry soil−1) of each lipid biomarker under ambient conditions (ambient), elevated nitrogen addition alone (+N), elevated temperature alone (+temperature), or both (+N and temperature), are shown. a = fungal to bacterial ratio [(average 16 : 1 ω5c, 18 : 1 ω9c, and 18 : 2 ω6,9c) divided by (average 15 : 0 iso, 15 : 0 anteiso, 16 : 0 2OH, 16 : 0 iso, 16 : 1 ω7c, 16 : 0 10 methyl, 17 : 0 iso, 17 : 0 anteiso, 17 : 0 cyclo, 18 : 1 ω5c, and 18 : 1 ω7c)]; b = Gram+ (Gram-positive bacterial, 15 : 0 iso) biomarker. Error bars represent one standard error. Letters represent a significant difference at P < 0.05 and letters with a star represent a significant difference from the letter ‘a’ at P < 0.1. In this figure, treatments were only compared across all years (left hand panel) or within each year (right hand panel) but not between years. Significance of these within-year or averaged effects was based on the full repeated measures model including all 6 years of data.
gcb2686-sup-0002-FigS2.pdfapplication/PDF91KFigure S2. Interactive effect between elevated CO2 and elevated temperature. The lipid biomass (nmol lipid g dry soil−1) of each lipid biomarker under ambient conditions (ambient), elevated CO2 (+CO2) alone, elevated temperature alone (+temperature), or both (+CO2 and temperature), are shown. a = lipid biomass (total lipid biomass, sum of lipids less than or equal to 20 carbons long); b = AMF (arbuscular mycorrhizal fungal, 16 : 1 ω5c) biomarker; c = Gram− (Gram-negative bacterial, 16 : 1 ω7c) biomarker. Error bars represent one standard error. Letters represent a significant difference at P < 0.05 and letters with a star represent a significant difference from the letter ‘a’ at P < 0.1. In this figure, treatments were only compared across all years (left hand panel) or within each year (right hand panel) but not between years. Significance of these within-year or averaged effects was based on the full repeated measures model including all 6 years of data.
gcb2686-sup-0003-FigS3.pdfapplication/PDF63KFigure S3. Interactive effect between elevated CO2 and nitrogen addition. The lipid biomass (nmol lipid g dry soil−1) of each lipid biomarker under ambient conditions (ambient), elevated CO2 (+CO2) alone, elevated nitrogen addition alone (+N), or both (+CO2 and N), are shown. a = fungal to bacterial ratio [(average 16 : 1 ω5c, 18 : 1 ω9c, and 18 : 2 ω6,9c) divided by (average 15 : 0 iso, 15 : 0 anteiso, 16 : 0 2OH, 16 : 0 iso, 16 : 1 ω7c, 16 : 0 10 methyl, 17 : 0 iso, 17 : 0 anteiso, 17 : 0 cyclo, 18 : 1 ω5c, and 18 : 1 ω7c)]; b = AMF (arbuscular mycorrhizal fungal, 16 : 1 ω5c) biomarker. Error bars represent one standard error. Letters represent a significant difference at P < 0.05 and letters with a star represent a significant difference from the letter ‘a’ at P < 0.1. In this figure, treatments were only compared across all years (left hand panel) or within each year (right hand panel) but not between years. Significance of these within-year or averaged effects was based on the full repeated measures model including all 6 years of data.

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